Coat-tether interaction in Golgi organization.

Molecular Biology of the Cell
Yusong GuoAdam D Linstedt

Abstract

Biogenesis of the Golgi apparatus is likely mediated by the COPI vesicle coat complex, but the mechanism is poorly understood. Modeling of the COPI subunit betaCOP based on the clathrin adaptor AP2 suggested that the betaCOP C terminus forms an appendage domain with a conserved FW binding pocket motif. On gene replacement after knockdown, versions of betaCOP with a mutated FW motif or flanking basic residues yielded a defect in Golgi organization reminiscent of that occurring in the absence of the vesicle tether p115. Indeed, betaCOP bound p115, and this depended on the betaCOP FW motif. Furthermore, the interaction depended on E(19)E(21) in the p115 head domain and inverse charge substitution blocked Golgi biogenesis in intact cells. Finally, Golgi assembly in permeabilized cells was significantly reduced by inhibitors containing intact, but not mutated, betaCOP FW or p115 EE motifs. Thus, Golgi organization depends on mutually interacting domains in betaCOP and p115, suggesting that vesicle tethering at the Golgi involves p115 binding to the COPI coat.

References

Jan 17, 1995·Proceedings of the National Academy of Sciences of the United States of America·S K SappersteinM G Waters
Sep 4, 1997·Nature·J F PresleyJ Lippincott-Schwartz
Apr 18, 1998·The Journal of Cell Biology·B SönnichsenG Warren
Jul 22, 1998·Proceedings of the National Academy of Sciences of the United States of America·A GodiM A De Matteis
Aug 4, 1999·Proceedings of the National Academy of Sciences of the United States of America·L M TraubD H Fremont
Dec 22, 1999·The Journal of Cell Biology·C AlvarezE Sztul
Apr 25, 2000·The Journal of Biological Chemistry·R V FuciniM Stamnes
Aug 16, 2000·The EMBO Journal·D J OwenP R Evans
Nov 14, 2001·The Journal of Cell Biology·T H WardJ Lippincott-Schwartz
Feb 21, 2002·Molecular Biology of the Cell·Raymond V FuciniMark Stamnes
Apr 18, 2002·Proceedings of the National Academy of Sciences of the United States of America·Francesco ParlatiJames E Rothman
Jul 11, 2003·The EMBO Journal·Meik DilcherGabriele Fischer von Mollard
Jul 24, 2003·The Journal of Cell Biology·Vangelis Kondylis, Catherine Rabouille
Aug 2, 2003·Proceedings of the National Academy of Sciences of the United States of America·Lena BurriTrevor Lithgow
Oct 21, 2003·Molecular Biology of the Cell·Sapna Puri, Adam D Linstedt
Dec 24, 2003·Traffic·Peter J WatsonDavid J Owen
Jan 23, 2004·Proceedings of the National Academy of Sciences of the United States of America·Manojkumar A Puthenveedu, Adam D Linstedt
Jan 28, 2004·Cell·Juan S Bonifacino, Benjamin S Glick
Mar 19, 2004·The EMBO Journal·Hidenori HiroseMitsuo Tagaya
Oct 12, 2004·Annual Review of Cell and Developmental Biology·David J OwenPhilip R Evans

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Citations

Mar 19, 2009·Nature Reviews. Molecular Cell Biology·Victor W HsuJia-Shu Yang
Mar 17, 2010·The Journal of Biological Chemistry·Collin Bachert, Adam D Linstedt
May 4, 2011·The Journal of Biological Chemistry·Andreea E RadulescuDennis Shields
Mar 21, 2009·The Journal of Biological Chemistry·Akina SaitohKazuhisa Nakayama
Mar 20, 2010·Molecular Biology of the Cell·Kaitlyn M DykstraTina H Lee
Oct 4, 2011·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Andreea E Radulescu, Dennis Shields
Aug 5, 2011·Cold Spring Harbor Perspectives in Biology·Daniel Kümmel, Karin M Reinisch
Jul 23, 2013·PLoS Computational Biology·Iaroslav Ispolatov, Anne Müsch
Feb 28, 2009·PloS One·Harald StrieglUdo Heinemann
Feb 4, 2010·PloS One·Harald StrieglUdo Heinemann
Mar 9, 2010·PloS One·Jonathon L BurmanPeter S McPherson
Jul 7, 2009·Annual Review of Cell and Developmental Biology·I-Mei Yu, Frederick M Hughson
Jul 8, 2009·The Journal of Cell Biology·Debrup SenguptaAdam D Linstedt
Apr 3, 2012·Progress in Neurobiology·Cecilia BucciCinzia Progida
Nov 6, 2009·FEBS Letters·Elizabeth Sztul, Vladimir Lupashin
Mar 26, 2016·Frontiers in Cell and Developmental Biology·Pak-Yan P Cheung, Suzanne R Pfeffer
Sep 17, 2011·Angewandte Chemie·Yanqiang ZhangJean'ne M Shreeve
Dec 17, 2009·Traffic·Fabiola MavillardAngel Velasco
Dec 2, 2009·Traffic·Eva KoeglerHans-Peter Hauri
Apr 25, 2012·The International Journal of Biochemistry & Cell Biology·Armen PetrosyanPi-Wan Cheng
Sep 21, 2012·Bioarchitecture·Robert GrabskiElizabeth Sztul
Jul 21, 2010·Seminars in Cell & Developmental Biology·Cortney G Angers, Alexey J Merz
Dec 4, 2014·Biochimica Et Biophysica Acta·Vinitha GanesanJonathan Minden
Apr 4, 2017·The FEBS Journal·Catherine DuclosJean-Bernard Denault
Mar 1, 2013·Molecular Biology of the Cell·Hanhui Ma, Thoru Pederson
Oct 21, 2011·Physiology·Tomasz Szul, Elizabeth Sztul
Jan 14, 2016·Oncotarget·Claudia CompagnucciEnrico Bertini
Jun 26, 2020·Molecular Biology of the Cell·Genki AmanoTaiichi Katayama
Sep 26, 2019·Frontiers in Cell and Developmental Biology·Yan HuangYusong Guo
May 1, 2020·Frontiers in Plant Science·Judit Sánchez-SimarroMaría Jesús Marcote
Feb 26, 2020·International Journal of Molecular Sciences·Manikandan VeerabaguChristiaan van der Schoot
Dec 19, 2019·Cells·Cole P FrisbieArmen Petrosyan
Mar 15, 2018·Journal of Cell Science·Eric C Arakel, Blanche Schwappach

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