Coated chitosan nanoparticles encapsulating caspase 3 activator for effective treatment of colorectral cancer

Drug Delivery and Translational Research
Aakanchha JainDharm Veer Kohli

Abstract

Cancer is the second leading cause of death worldwide, the deaths are projected to continue rising, with an estimated 12 million deaths in 2030. The aim of the present investigation is to prepare and compare the uncoated (U-CH NP) and eudragit S 100-coated (E-U-CH NP) chitosan nanoparticles encapsulating a caspase 3 activator (UCN 01), by ionic gelation method. The prepared formulations were studied for various parameters like particles size, zeta potential, transmission electron microscopy, atomic force microscopy, in vitro release study, ex vivo study using Caco 2 colon cancer cell line, and in vivo studies. The particle size and zeta potential of developed formulation was found to be particle size of 168 ± 3.7 nm and +35.8 ± 3.7 for U-CH NP and 265 ± 4.1 nm and +22.3 ± 1.1 for E-U-CH NP. TEM and AFM images revealed that U-CH NPs were round in shape and smoother at surface as compared to E-U-CH NP which have irregular surface due to coating. The E-U-CH NP showed better in vitro release than uncoated formulation in SCF (pH 6.8) than in SGF (pH 1.2). The cytotoxicity was performed by MTT assay. U-CH NP showed enhanced cytotoxicity as compared to blank (without drug) formulation. There was an increase in caspase 3 activity of U-...Continue Reading

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Citations

Apr 4, 2019·Artificial Cells, Nanomedicine, and Biotechnology·Aakanchha JainDharam Veer Kohli
Sep 27, 2016·Expert Opinion on Drug Delivery·Chintan BhavsarAbdelwahab Omri
Mar 11, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Somayeh RezaeiMarjan Motiei
Oct 30, 2020·Drug Design, Development and Therapy·Nasrul WathoniMuchtaridi Muchtaridi
Mar 20, 2021·Macromolecular Bioscience·Asim MushtaqLisbeth Grøndahl

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