PMID: 9448287Mar 14, 1998Paper

Coatomer, but not P200/myosin II, is required for the in vitro formation of trans-Golgi network-derived vesicles containing the envelope glycoprotein of vesicular stomatitis virus

Proceedings of the National Academy of Sciences of the United States of America
J P SimonD D Sabatini

Abstract

Using a cytosol and nucleotide dependent assay that we previously developed, we have investigated the requirement for coat proteins in the in vitro production of trans-Golgi network (TGN)-derived vesicles from a Madin-Darby canine kidney (MDCK) cell Golgi fraction that contains the 35S-labeled, terminally glycosylated, envelope glycoprotein of vesicular stomatitis virus (VSV-G) accumulated in the TGN. We found that the TGN-derived vesicles, like those involved in intra-Golgi transport and in retrograde transport to the endoplasmic reticulum, contain a coatomer coat and that coatomer is required for their formation. Thus, after they are produced with GTPgammaS, the coated vesicles could be captured on beads containing anticoatomer antibody. Moreover, a cytosolic protein fraction depleted of coatomer could not support vesicle formation but it did so after purified coatomer was added. We also determined that P200/myosin II does not play an essential role in the in vitro generation of TGN-derived vesicles. Thus, removal of this protein from the cytosol, by differential salt precipitation or binding to phalloidin-induced actin filaments, had no effect on vesicle generation. Nevertheless, immunodepletion of cytosol using the anti-P20...Continue Reading

References

Jan 1, 1992·Methods in Enzymology·M G WatersJ E Rothman
Apr 1, 1992·The Journal of Cell Biology·N NarulaJ L Stow
Oct 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·J SalameroK E Howell
Dec 1, 1986·The Journal of Cell Biology·V J Allan, T E Kreis
Mar 28, 1995·Proceedings of the National Academy of Sciences of the United States of America·N Narula, J L Stow
Apr 1, 1995·The Journal of Cell Biology·G Miesenböck, J E Rothman
Dec 3, 1993·Cell·J OstermannJ E Rothman
Dec 1, 1995·Cell·J A WhitneyI Mellman
Mar 15, 1996·Science·R Schekman, L Orci
Apr 12, 1996·Science·J E Rothman, F T Wieland
Mar 22, 1996·The Journal of Biological Chemistry·D SheffI Mellman
Jun 1, 1996·Molecular Biology of the Cell·E IkonenK Simons
Nov 29, 1996·The Journal of Biological Chemistry·M Lowe, T E Kreis
Jul 28, 1997·The Journal of Cell Biology·A MüschE Rodriguez-Boulan
Aug 1, 1997·Current Opinion in Cell Biology·P Cosson, F Letourneur

❮ Previous
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Citations

Nov 24, 2001·Trends in Cell Biology·W J Nelson, C Yeaman
Jun 22, 2010·Nature Cell Biology·S Miserey-LenkeiA Echard
Mar 21, 2008·Nature Reviews. Molecular Cell Biology·Maria Antonietta De Matteis, Alberto Luini
May 8, 1999·Molecular Biology of the Cell·G RaposoE Coudrier
Nov 18, 2003·Molecular Biology of the Cell·Tatsuru Togo, Richard A Steinhardt
Mar 9, 2002·Journal of Virology·Hans van LeeuwenPeter O'Hare
Sep 16, 1998·Proceedings of the National Academy of Sciences of the United States of America·J P SimonD D Sabatini
Nov 17, 2010·PloS One·Lukas SchollenbergerWilhelm W Just
Oct 2, 2002·FEBS Letters·Tounsia Aït Slimane, Dick Hoekstra
Oct 19, 1999·Microscopy Research and Technique·A S DePina, G M Langford
Oct 2, 2004·The Journal of Biological Chemistry·Mark A EllisOra A Weisz
Jul 17, 2007·Cell Motility and the Cytoskeleton·Ana S DePinaGeorge M Langford
Mar 9, 2005·Cell Motility and the Cytoskeleton·Karl R Fath
Apr 10, 1999·The Journal of Biological Chemistry·K HeimannJ L Stow
Jan 29, 1999·Physiological Reviews·C YeamanW J Nelson
Jun 20, 2000·The Journal of Biological Chemistry·M E MartinA Velasco
Feb 24, 2001·Traffic·R I Tuxworth, M A Titus
Oct 29, 1998·Methods : a Companion to Methods in Enzymology·W L LingD Shields

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