Cobalamin is present in cells of non-tuberculous mycobacteria, but not in Mycobacterium tuberculosis.

Scientific Reports
Alina MiniasJarosław Dziadek

Abstract

Cobalamin (vitamin B12) is a structurally complex molecule that acts as a cofactor for enzymes and regulates gene expression through so-called riboswitches. The existing literature on the vitamin B12 synthesis capacity in Mycobacterium tuberculosis is ambiguous, while in non-tuberculous mycobacteria (NTM) is rather marginal. Here we present the results of our investigation into the occurrence of vitamin B12 in mycobacteria. For detection purposes, immunoassay methods were applied to cell lysates of NTM and M. tuberculosis clinical and laboratory strains grown under different conditions. We show that whereas vitamin B12 is present in cells of various NTM species, it cannot be evidenced in strains of differently cultured M. tuberculosis, even though the genes responsible for vitamin B12 synthesis are actively expressed based on RNA-Seq data. In summary, we conclude that the production of vitamin B12 does occur in mycobacteria, with the likely exception of M. tuberculosis. Our results provide direct evidence of vitamin B12 synthesis in a clinically important group of bacteria.

References

Oct 13, 2001·Trends in Microbiology·J M Reyrat, D Kahn
Oct 7, 2003·Annual Review of Biochemistry·Ruma Banerjee, Stephen W Ragsdale
Jan 6, 2004·Nucleic Acids Research·Ali NahviRonald R Breaker
Aug 3, 2010·ACS Chemical Biology·Karl-Magnus LarssonPär Nordlund
Sep 29, 2011·The International Journal of Tuberculosis and Lung Disease : the Official Journal of the International Union Against Tuberculosis and Lung Disease·M KrawczykJ Dziadek
Mar 6, 2012·Nature Methods·Ben Langmead, Steven L Salzberg
Jan 22, 2013·Cell·Alexander Serganov, Evgeny Nudler
Feb 15, 2013·Open Biology·Krishnamoorthy GopinathDigby F Warner
Jan 1, 2014·Antimicrobial Agents and Chemotherapy·Aneta KuronJaroslaw Dziadek
Nov 18, 2015·BMC Genomics·Dmitriy V IgnatovArseny S Kaprelyants
Oct 21, 2016·PLoS Pathogens·Marissa B GuzzoLiem Nguyen
Feb 1, 2017·Microbial Cell Factories·Huan FangDawei Zhang
Aug 24, 2018·Current Opinion in Biotechnology·Carlos G Acevedo-RochaHans J Genee
Oct 18, 2019·Antioxidants & Redox Signaling·Parminder Singh MaviAshwani Kumar
Dec 15, 2019·Proceedings of the National Academy of Sciences of the United States of America·Jing LiMansour Mohamadzadeh
Jun 11, 2020·Nature Communications·Jean Claude Semuto NgabonzizaPhilip Supply
Jul 8, 2020·Science·Olga M SokolovskayaMichiko E Taga

❮ Previous
Next ❯

Software Mentioned

Geneious
Mycobrowser
Bowtie2 Geneious
Develve

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

Clinical Chemistry
V A TRUFANOV
Scandinavian Journal of Clinical and Laboratory Investigation
Anne-Mette HvasEbba Nexo
Journal of Pediatric Hematology/oncology
Rabia G SezerGül N Özdemir
American Family Physician
Robert C Langan, Kimberly J Zawistoski
© 2021 Meta ULC. All rights reserved