Cocaine- and amphetamine-regulated transcript in catecholamine and noncatecholamine presympathetic vasomotor neurons of rat rostral ventrolateral medulla

The Journal of Comparative Neurology
Kathleen J BurmanI J Llewellyn-Smith

Abstract

Presympathetic vasomotor adrenergic (C1) and nonadrenergic (non-C1) neurons in the rostral ventrolateral medulla (RVLM) provide the main excitatory drive to cardiovascular sympathetic preganglionic neurons in the spinal cord. C1 and non-C1 neurons contain cocaine- and amphetamine-regulated transcript (CART), suggesting that CART may be a common marker for RVLM presympathetic neurons. To test this hypothesis, we first used double-immunofluorescence staining for CART and tyrosine hydroxylase (TH) to quantify CART-immunoreactive (-IR) catecholamine and noncatecholamine neurons in the C1 region. Next, we quantified the proportion of CART-IR RVLM neurons that expressed Fos in response to a hypotensive stimulus, using peroxidase immunohistochemistry for Fos and dual immunofluorescence for CART and TH. Finally, we fluorescently detected CART immunoreactivity in electrophysiologically identified, juxtacellularly labeled RVLM presympathetic neurons. In the RVLM, 97% of TH-IR neurons were CART-IR, and 74% of CART-IR neurons were TH-IR. Nitroprusside infusion significantly increased the number of Fos-IR RVLM neurons compared with saline controls. In nitroprusside-treated rats, virtually all Fos/TH neurons in the RVLM were immunoreactive f...Continue Reading

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Citations

Jan 29, 2014·Peptides·Nishikant K SubhedarDadasaheb M Kokare
Sep 20, 2008·Nature Reviews. Neuroscience·G RoggeM J Kuhar
Mar 1, 2008·Clinical and Experimental Pharmacology & Physiology·Paul M PilowskyAnn K Goodchild
Aug 12, 2009·Journal of Chemical Neuroanatomy·Ruth L Stornetta
Jun 25, 2009·Journal of Chemical Neuroanatomy·Ann K Goodchild, Elizabeth A Moon
Jan 26, 2013·The Journal of Comparative Neurology·Lindsay M ParkerAnn K Goodchild
Jan 26, 2013·The Journal of Comparative Neurology·Romke BronJohn B Furness
Jun 13, 2006·Peptides·Niels Vrang
Aug 24, 2018·The Journal of Comparative Neurology·M SenthilkumaranI J Llewellyn-Smith
Feb 15, 2008·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Melissa M J FarnhamPaul M Pilowsky
Apr 4, 2009·Physiological Reviews·Richard KvetnanskyMiklos Palkovits
Apr 27, 2020·Brain Research·Zhi Yi Ong, Gavan P McNally

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