Cocaine analogue conjugated magnetic nanoparticles for labeling and imaging dopaminergic neurons.

Biomaterials Science
Mike JeonMiqin Zhang

Abstract

Molecular imaging of the dopamine transporter (DAT) with Positron Emission Tomography (PET) or Single Photon Emission Computed Tomography (SPECT) has been widely used in studies of neurological and psychiatric disorders. Nevertheless, there is a great interest in expanding molecular imaging to include magnetic resonance technology, because of the superior spatial resolution this technology may provide. Here we present a magnetic nanoparticle (NP) that specifically targets dopaminergic neurons and allows DAT imaging with magnetic resonance imaging (MRI). The nanoparticle (namely, NP-DN) is composed of an iron oxide core and a polyethylene glycol (PEG) coating to which a DAT specific dopaminergic neurolabeler (DN) is conjugated. NP-DN displayed long-term stability with favorable hydrodynamic size and surface charge suitable for in vivo application. In vitro studies showed NP-DN was non-toxic, displayed specificity towards DAT-expressing neurons, and demonstrated a 3-fold increase in DAT labeling over non-targeted NP. Our study shows NP-DN provides excellent contrast enhancement for MRI and demonstrates great potential for neuroimaging.

References

Aug 5, 1998·Proceedings of the National Academy of Sciences of the United States of America·J V Ferrer, J A Javitch
Aug 26, 1998·BioTechniques·A K Robbins, R A Horlick
Jan 15, 2000·The Journal of Biological Chemistry·N ChenJ B Justice
Aug 30, 2001·Proceedings of the National Academy of Sciences of the United States of America·H HastrupJ A Javitch
Nov 30, 2004·Nature Materials·Jongnam ParkTaeghwan Hyeon
Nov 7, 2008·Angewandte Chemie·Philip W MillerAntony D Gee
May 29, 2009·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Jacob EriksenUlrik Gether
Jun 17, 2010·The Journal of Biological Chemistry·Jacob EriksenUlrik Gether
Aug 4, 2011·Brain : a Journal of Neurology·Jeffrey L CummingsPaul Kemp
Jul 21, 2012·ACS Chemical Neuroscience·Oleg KovtunSandra J Rosenthal
Aug 21, 2014·Expert Opinion on Drug Delivery·Nghia P TruongThomas P Davis
Dec 10, 2014·Parkinsonism & Related Disorders·Fang Ba, W R Wayne Martin
Mar 13, 2015·Advanced Healthcare Materials·Qingxin MuMiqin Zhang
Apr 30, 2016·Stem Cells International·Michelle R Santoso, Phillip C Yang
May 22, 2016·Journal of Controlled Release : Official Journal of the Controlled Release Society·Cláudia SaraivaLiliana Bernardino
Sep 9, 2017·ACS Nano·Peter A ChiarelliMiqin Zhang
Dec 23, 2017·Journal of Controlled Release : Official Journal of the Controlled Release Society·Yiqun ZhouRoger M Leblanc
Sep 10, 2019·Chemical Society Reviews·Jie Kai TeeDavid Tai Leong

❮ Previous
Next ❯

Methods Mentioned

BETA
transmission
dynamic light scattering
fluorescence microscopy
flow cytometry
confocal microscopy

Software Mentioned

Paravision
FMRIB
OriginPro
Image J
ImageJ

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

World Journal of Diabetes
Jonathan KopelKenneth Nugent
The Journal of Neuroscience : the Official Journal of the Society for Neuroscience
Jacob EriksenUlrik Gether
Journal of the American Geriatrics Society
C J Gilleard
© 2022 Meta ULC. All rights reserved