Cocaine and metabolite concentrations in DBS and venous blood after controlled intravenous cocaine administration

Bioanalysis
Kayla N EllefsenMarilyn A Huestis

Abstract

DBS are an increasingly common clinical matrix. Sensitive and specific methods for DBS and venous blood cocaine and metabolite detection by LC-HRMS and 2D GC-MS, respectively, were validated to examine correlation between concentrations following controlled intravenous cocaine administration. Linear ranges from 1 to 200 µg/l were achieved, with acceptable bias and imprecision. Authentic matched specimens' (392 DBS, 97 venous blood) cocaine and benzoylecgonine concentrations were qualitatively similar, but DBS had much greater variability (21.4-105.9 %CV) and were lower than in blood. DBS offer advantages for monitoring cocaine intake; however, differences between capillary and venous blood and DBS concentration variability must be addressed.

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Citations

Feb 7, 2016·Forensic Science International : Synergy·Kayla N EllefsenMarilyn A Huestis
Dec 25, 2016·Analytical and Bioanalytical Chemistry·Tiago RosadoEugenia Gallardo
Jun 18, 2019·Journal of Forensic Sciences·Ana Laura Bemvenuti JacquesRenata Pereira Limberger
Sep 7, 2019·Mass Spectrometry Reviews·Benjamin S FreyAbraham K Badu-Tawiah
Jan 20, 2020·Analytical and Bioanalytical Chemistry·Hyo-Jin KimHee-Gyoo Kang
Mar 1, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Roberto MandrioliMichele Protti
Jul 5, 2021·International Journal of Legal Medicine·Hyo-Jin KimHee-Gyoo Kang

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Methods Mentioned

BETA
pharmacotherapies

Software Mentioned

Prism
Thermo TraceFinder Clinical Research
GraphPad

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