Coccidioides immitis antigen 2: analysis of gene and protein

Gene
Y ZhuR A Cox

Abstract

Antigen 2 is a glycosylated protein present in the cell walls of the dimorphic fungus Coccidioides immitis. Using oligodeoxyribonucleotide (oligo) primers based on the sequences of Ag2 cDNA, the gene encoding Ag2 was cloned from genomic DNA derived from the mycelial phase of C. immitis by PCR. Nucleotide (nt) sequence analyses showed a 582 base pair (bp) ORF disrupted by two introns which are 78 bp and 101 bp long. The deduced primary translation product consists of 194 amino acids (aa), contains an N-terminal putative signal sequence to allow transport into the endoplasmic reticulum, and a C-terminal putative signal sequence to enable a GPI anchor addition. Putative GPI anchor/cleavage site and O-glycosylation sites, as well as phosphorylation and myristoylation sites are also present. On the basis of these analyses, we predict that a prepro-Ag2 undergoes a post-translational modification to yield the mature glycosylated Ag2 protein which is anchored on the extracellular plasma membrane of mycelial and spherule-phase cells.

References

Dec 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·F SangerA R Coulson
Mar 1, 1978·Survey of Ophthalmology·K A ZakkaW J Brown
Jul 1, 1991·Infection and Immunity·K O DuggerJ H Law
Sep 24, 1990·Biochimica Et Biophysica Acta·L A Pinna
Oct 5, 1990·Journal of Molecular Biology·S F AltschulD J Lipman
Mar 1, 1988·Computer Applications in the Biosciences : CABIOS·B A Jameson, H Wolf
Feb 1, 1989·The Journal of Cell Biology·M Kozak
Mar 15, 1989·The Biochemical Journal·R J Grand
May 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·A SetteH M Grey
Jun 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·J L PaluhC Yanofsky
Jan 1, 1988·Annual Review of Biochemistry·D A TowlerL Glaser
Jan 1, 1987·Critical Reviews in Biotechnology·J Rambosek, J Leach
Jun 11, 1986·Nucleic Acids Research·G von Heijne
Jul 11, 1984·Nucleic Acids Research·M E Watson
May 5, 1982·Journal of Molecular Biology·J Kyte, R F Doolittle
Jan 3, 1980·The New England Journal of Medicine·M L Sievers
Dec 1, 1993·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·D Salkin, A Said
Jan 1, 1993·Annual Review of Biochemistry·P T Englund
Jan 17, 1996·Biochemical and Biophysical Research Communications·K O DuggerJ N Galgiani

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Citations

Mar 14, 2003·Trends in Biochemical Sciences·Resham D KulkarniRalph A Dean
Jul 31, 2004·Medical Mycology·G T ColeC Y Hung
Oct 19, 2004·Clinical Microbiology Reviews·Rebecca A Cox, D Mitchell Magee
Jun 18, 2002·Infection and Immunity·Chengyong JiangRebecca A Cox
Oct 16, 2010·F1000 Medicine Reports·Neil M Ampel
Mar 10, 2007·Annals of the New York Academy of Sciences·Suzanne M JohnsonRichard F Hector
Nov 28, 2002·International Journal of Medical Microbiology : IJMM·Raed O AbuodehGene M Scalarone
Feb 21, 2009·PloS One·Luciana Pereira RuasMaria-Cristina Roque-Barreira
Dec 17, 2014·Journal of Immunological Methods·Krupa Arun NavalkarPhillip Stafford
Jan 7, 2016·PLoS Neglected Tropical Diseases·Felipe Souto AraújoMaristela Pereira
Jul 23, 1998·Infection and Immunity·T N KirklandJ N Galgiani

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