Cochlear outer hair cell horizontal top connectors mediate mature stereocilia bundle mechanics

Science Advances
Alexander X Cartagena-RiveraRichard S Chadwick

Abstract

Outer hair cell (OHC) stereocilia bundle deflection opens mechanoelectrical transduction channels at the tips of the stereocilia from the middle and short rows, while bundle cohesion is maintained owing to the presence of horizontal top connectors. Here, we used a quantitative noncontact atomic force microscopy method to investigate stereocilia bundle stiffness and damping, when stimulated at acoustic frequencies and nanometer distances from the bundle. Stereocilia bundle mechanics were determined in stereocilin-deficient mice lacking top connectors and with detached tectorial membrane (Strc-/-/Tecta-/- double knockout) and heterozygous littermate controls (Strc+/-/Tecta-/-). A substantial decrease in bundle stiffness and damping by ~60 and ~74% on postnatal days P13 to P15 was observed when top connectors were absent. Additionally, we followed bundle mechanics during OHC top connectors development between P9 and P15 and quantified the observed increase in OHC bundle stiffness and damping in Strc+/-/Tecta-/- mice while no significant change was detected in Strc-/-/Tecta-/- animals.

References

Aug 22, 1992·Proceedings. Biological Sciences·C J KrosG P Richardson
Dec 22, 1992·Proceedings. Biological Sciences·I J RussellG P Richardson
Jan 11, 1985·Science·W E BrownellY de Ribaupierre
Jul 1, 1984·Hearing Research·D Strelioff, A Flock
Jul 1, 1980·The Journal of Cell Biology·L G TilneyM J Mulroy
Dec 8, 1994·The Journal of Comparative Neurology·J A KaltenbachT H Simpson
Nov 1, 1996·Anatomy and Embryology·J RuedaD J Lim
Mar 19, 2005·The Journal of Comparative Neurology·Richard J GoodyearGuy P Richardson
Apr 7, 2005·Hearing Research·Marcus MüllerJean W T Smolders
Jun 15, 2006·The Journal of Membrane Biology·A J RicciS M Van Netten
Jun 15, 2007·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Nicolas MichalskiChristine Petit
Aug 5, 2008·Biophysical Journal·Jong-Hoon Nam, Robert Fettiplace
Oct 14, 2008·Nature·Elisabeth VerpyChristine Petit
Jul 9, 2010·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·K Domenica Karavitaki, David P Corey
Nov 16, 2010·Annual Review of Physiology·Guy P RichardsonChristine Petit
Dec 18, 2010·The Journal of Comparative Neurology·Elisabeth VerpyChristine Petit
Feb 14, 2012·Proceedings of the National Academy of Sciences of the United States of America·Andrei S KozlovA J Hudspeth
Oct 4, 2012·Nature Communications·Pierre HakizimanaAnders Fridberger
Oct 19, 2013·Physiological Reviews·Paul AvanChristine Petit
May 7, 2014·Proceedings of the National Academy of Sciences of the United States of America·Volker BormuthPascal Martin
Aug 7, 2014·Nature Reviews. Neuroscience·A J Hudspeth
Oct 19, 2017·Nature Communications·Alexander X Cartagena-RiveraRichard S Chadwick

❮ Previous
Next ❯

Citations

May 3, 2020·Proceedings of the National Academy of Sciences of the United States of America·Woongsu HanChul Hoon Kim
Dec 14, 2021·Frontiers in Cell and Developmental Biology·Katharine K MillerNicolas Grillet

❮ Previous
Next ❯

Methods Mentioned

BETA
atomic force microscopy
AFM
scanning electron microscopy
fluorescence microscopy
genotyping

Software Mentioned

Nanoscope Analysis
GraphPad
ImageJ
MATLAB
GraphPad Prism

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.