Cockroach allergens Per a 3 are oligomers

Developmental and Comparative Immunology
Beatrice MindykowskiHeinz Decker

Abstract

Allergens from cockroaches cause major asthma-related health problems worldwide. Among them Per a 3 belongs to the most potent allergens. Although the sequences of some members of the Per a 3-family are known, their biochemical and biophysical properties have not been investigated. Here we present for the first time a thorough structural characterization of these allergens, which have recently been tested to induce an increase of allergy specific indicators in blood of Europeans. We isolated two Per a 3 isoforms, which occur freely dissolved in the hemolymph as hexamers with molecular masses of 465+/-25kDa (P II) and 512+/-25kDa (P I). Their sedimentation coefficients (S(20,W)) were determined to be 17.4+/-0.7 S (P II) and 19.0+/-0.9 S (P I), respectively. Sequence analysis revealed that P II consists of two subunit types known as allergens Per a 3.01 and Per a 3.0201, while PI consists of a new allergenic subunit type designated as Per a 3.03. A 3D model of the hexameric allergen Per a 3 was obtained by homology modelling. Almost all of the recently predicted 11 putative antigenic peptides and reported IgE-epitopes could be located on the surface of the hexamer, thus being freely accessible in the hexameric structure of the na...Continue Reading

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Citations

Feb 25, 2014·Current Allergy and Asthma Reports·L Karla ArrudaAnna Pomés
Apr 8, 2014·Mediators of Inflammation·Ji-Fu WeiShaoheng He
Jan 29, 2014·Annals of Allergy, Asthma & Immunology : Official Publication of the American College of Allergy, Asthma, & Immunology·Taruna KhuranaJay E Slater
Aug 14, 2013·The Journal of Allergy and Clinical Immunology·Jay PortnoyUNKNOWN Joint Task Force on Practice Parameters
Apr 20, 2017·Current Allergy and Asthma Reports·Anna PomésL Karla Arruda
Nov 15, 2016·International Journal of Molecular Medicine·Xunliang TongJi-Fu Wei
May 31, 2017·Archives of Insect Biochemistry and Physiology·Ganesh ArumugamJanarthanan Sundaram
Mar 2, 2021·Frontiers in Immunology·Anna PomésAlessandro Sette
May 7, 2020·The World Allergy Organization Journal·Luis CaraballoAlexander Karaulov

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