CoCl2 induces apoptosis via a ROS-dependent pathway and Drp1-mediated mitochondria fission in periodontal ligament stem cells

American Journal of Physiology. Cell Physiology
Yuting HeHaiyang Yu

Abstract

Oxygen deficiency is associated with various oral diseases, including chronic periodontitis, age-related alveolar bone loss, and mechanical stress-linked cell injury from orthodontic appliances. Nevertheless, our understanding of the impact of hypoxia on periodontal tissues and its biochemical mechanism is still rudimentary. The purpose of this research was to elucidate the effects of hypoxia on the apoptosis of human periodontal ligament stem cells (PDLSCs) in vitro and the underlying mechanism. Herein, we showed that cobalt chloride (CoCl2) triggered cell dysfunction in human PDLSCs in a concentration-dependent manner and resulted in cell apoptosis and oxidative stress overproduction and accumulation in PDLSCs. In addition, CoCl2 promoted mitochondrial fission in PDLSCs. Importantly, CoCl2 increased the expression of dynamin-related protein 1 (Drp1), the major regulator in mitochondrial fission, in PDLSCs. Mitochondrial division inhibitor-1, pharmacological inhibition of Drp1, not only inhibited mitochondrial fission but also protected against CoCl2-induced PDLSC dysfunction, as shown by increased mitochondrial membrane potential, increased ATP level, reduced reactive oxygen species (ROS) level, and decreased apoptosis. Furth...Continue Reading

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Citations

Mar 19, 2019·Oxidative Medicine and Cellular Longevity·Chen-Chen CaiZhen-Lang Lin
Mar 1, 2020·Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry·Hana CrlikovaJana Kasparkova
Oct 28, 2019·International Journal of Molecular Sciences·Ssu-Yu ChenShih-Ming Huang
Nov 24, 2020·The International Journal of Biochemistry & Cell Biology·Suryakant NitureDeepak Kumar
Sep 27, 2020·Annals of the New York Academy of Sciences·Jingjing ZhengLing Gao
Nov 13, 2020·Frontiers in Cell and Developmental Biology·Lin RenJuan Xia
Dec 18, 2020·Frontiers in Chemistry·Lihua SuiKelong Ai
May 10, 2021·Toxicon : Official Journal of the International Society on Toxinology·Man ZhaoJinfeng Cui
Jul 30, 2021·Ecotoxicology and Environmental Safety·Shiyao SunJunhua Yang
Jan 18, 2022·Journal of Periodontal Research·Xiaochi ChangShuai Wang

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Methods Mentioned

BETA
flow cytometry
Assay
Confocal microscopy
GTPase

Software Mentioned

ImageJ
Statview
NIH ImageJ

Related Concepts

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Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis