Coculture with cumulus cells improves maturation of mouse oocytes denuded of the cumulus oophorus: observations of nuclear and cytoplasmic events

Fertility and Sterility
Li GeJing-He Tan

Abstract

To study the mechanisms by which cumulus cells (CCs) promote oocyte maturation by observing the effect of removing the cumulus oophorus on nuclear and cytoplasmic events during in vitro maturation. Experimental animal study. Academic institution. Mice of the Kun-ming breed. Cumulus-free oocytes were cultured alone (DOs) or with a CC monolayer (coDOs), and the nuclear and cytoplasmic events were compared with those of oocytes matured in vivo or in vitro with the cumulus intact (COCs). Nuclear progression, spindle assembly, behavior of cortical granules (CGs) and mitochondria, levels of glutathione (GSH), and dynamics of maturation-promoting factor (MPF) activity during oocyte maturation under different conditions. Cumulus removal increased MPF activity and accelerated the transition from the G2 to the M phase and the redistribution of CGs. Spindle assembly and mitochondrial congregation were impaired. In addition, removal of the cumulus caused a precocious exocytosis of CGs, leading to zona hardening and reduced penetrability of oocytes by sperm. After DOs were matured on the CC monolayer, however, these parameters were much improved, and the DOs acquired characteristics closer to those of cumulus-invested oocytes matured in viv...Continue Reading

References

May 1, 1991·Molecular Reproduction and Development·G GaleatiM Mattioli
Apr 1, 1984·Developmental Biology·A C Schroeder, J J Eppig
Mar 1, 1995·Molecular Reproduction and Development·L ZhangR A Godke
Dec 1, 1995·Molecular Reproduction and Development·D G de MatosH Baldassarre
Jul 1, 1996·Journal of Reproduction and Fertility·S CecconiR Colonna
Aug 1, 1996·Molecular Human Reproduction·C FurgerM Pouchelet
Sep 22, 1999·European Journal of Biochemistry·P BernardiF Di Lisa
Jun 27, 2001·Reproduction : the Official Journal of the Society for the Study of Fertility·Q Y SunH Schatten
Feb 9, 2002·Molecular Reproduction and Development·Sofie TangheAart de Kruif
Apr 12, 2005·Reproduction in Domestic Animals = Zuchthygiene·P WongsrikeaoT Otoi
Jul 1, 2005·Biology of Reproduction·Yi-Liang MiaoJing-He Tan

❮ Previous
Next ❯

Citations

Apr 1, 2010·Reproduction, Fertility, and Development·E C CurnowE S Hayes
Mar 30, 2011·Human Reproduction Update·Maurizio ZuccottiSilvia Garagna
May 31, 2014·PloS One·Guang-Li ZhangGuang-Jian Jiang
Nov 26, 2008·Reproduction : the Official Journal of the Society for the Study of Fertility·Jun-Zuo WangJing-He Tan
Oct 3, 2013·Stem Cells and Development·Jian MaoLin Liu
Feb 2, 2013·Zygote : the Biology of Gametes and Early Embryos·Xue-Ming ZhaoHua-Bin Zhu
Apr 17, 2014·BioMed Research International·In-Sul Hwang, Shinichi Hochi
Apr 29, 2014·Taiwanese Journal of Obstetrics & Gynecology·Yu-Hung LinChii-Ruey Tzeng
Jul 11, 2009·Fertility and Sterility·Jin-Hua LuJing-He Tan
May 30, 2014·Zygote : the Biology of Gametes and Early Embryos·Xue-Ming ZhaoHua-Bin Zhu
Oct 1, 2016·Reproduction in Domestic Animals = Zuchthygiene·M G MorselliG C Luvoni
Mar 3, 2018·Journal of Assisted Reproduction and Genetics·M G Da BroiP A Navarro
Jul 13, 2017·Environmental Science and Pollution Research International·Juan Mateo AnchordoquyCecilia C Furnus
Mar 20, 2021·Reproductive Biomedicine Online·Caroline M Da LuzPaula A Navarro
Dec 31, 2017·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Jin LiuWenchang Zhang

❮ Previous
Next ❯

Related Concepts

Related Feeds

Cell Checkpoints & Regulators

Cell cycle checkpoints are a series of complex checkpoint mechanisms that detect DNA abnormalities and ensure that DNA replication and repair are complete before cell division. They are primarily regulated by cyclins, cyclin-dependent kinases, and the anaphase-promoting complex/cyclosome. Here is the latest research.