Cocultures of metastatic and host immune cells: selective effects of NAMI-A for tumor cells

Cancer Immunology, Immunotherapy : CII
M BacacS Pacor

Abstract

The effects of NAMI-A, [H2im][trans-RuCl4(dmso-S)(Him)], a new metal-based agent for treating tumor metastases, have been investigated in vitro on splenocytes, ConA- or LPS-activated T and B lymphoblasts, and thymocytes. Splenocytes and thymocytes exposed for 1 h to 0.01-0.1-mM NAMI-A do not change their mitochondrial functionality, cell cycle distribution, protein synthesis, and CD44 expression in comparison to untreated control samples. Instead, mitochondrial functionality increased 24 h after treatment in a fraction of splenocytes. The same treatment reduced mitochondrial functionality and S phase of the cell cycle in T and B blasts (already after 1 h treatment) and reduced CD44 expression on B blasts, 24 h after treatment. On cocultures of splenocytes and metastatic cells (metGM) (1:1), NAMI-A induces a selective depolarization of mitochondrial membrane potential of metGM cells, while it stimulates splenocytes (mainly lymphocytes), as shown by the increase of the S phase, nitric oxide production, and adhesion onto metastatic cells. This, in turn, reduces the number of metastatic cells and results in the increased ratio between splenocytes and metGM in favor of diploid cells (doubling from one to two). Rosetting of leukocyte...Continue Reading

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Citations

Dec 25, 2015·Dalton Transactions : an International Journal of Inorganic Chemistry·Eswaran JayanthiNallasamy Dharmaraj
Jul 16, 2015·Dalton Transactions : an International Journal of Inorganic Chemistry·Michael I Webb, Charles J Walsby
Apr 2, 2015·Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry·Miljana MomcilovicStanislava Stosic-Grujicic
Aug 1, 2018·Dalton Transactions : an International Journal of Inorganic Chemistry·Silvia CiambellottiLuigi Messori
Jan 7, 2011·Dalton Transactions : an International Journal of Inorganic Chemistry·Michael I Webb, Charles J Walsby
Apr 29, 2021·Inorganic Chemistry·Pran Gobinda NandiAkshai Kumar
Nov 30, 2018·Chemical Reviews·Bernhard EnglingerWalter Berger

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