Cocultures of rat sensorimotor cortex and spinal cord slices to investigate corticospinal tract sprouting

Spine
Stavros I StavridisNils P Hailer

Abstract

Experimental study of corticospinal axonal sprouting in an organotypic slice culture model. To develop an in vitro model that simplifies the study of various factors regulating neuronal regeneration. Spinal cord injury leads to permanent neurologic damage, mainly due to the inability of the adult central nervous system to regenerate. Much attention has been focused on promoting axonal regeneration and sprouting, either by exogenous administration of various neurotrophic factors or by the antagonization of factors inhibiting regeneration. An in vitro system that allows coculture of slices from rat sensorimotor cortex and spinal cord (p4) was established. Two groups of cultures were investigated: In the first group, intact spinal cord slices were cultured adjacent to sensorimotor cortex slices, while in the second group the spinal cord slices were sagittally cut into halves, with the sectioned interface placed directly adjacent to the sensorimotor cortex, to prevent the spinal white matter from interference. Each group was further divided into 2 subgroups: The neurotrophin-3 (NT-3) group, where the culture medium contained 50 ng/mL NT-3 and the control group treated with normal culture medium. Sensorimotor cortex pyramidal neuron...Continue Reading

References

Apr 1, 1996·Physiological Reviews·M E Schwab, D Bartholdi
Jun 1, 1996·The European Journal of Neuroscience·K M Giehl, W Tetzlaff
Jul 15, 1996·The Journal of Comparative Neurology·M C Halloran, K Kalil
Nov 5, 1997·Trends in Neurosciences·B H GähwilerS M Thompson
Mar 31, 1998·Hippocampus·A KlugeR Nitsch
Mar 27, 2001·The European Journal of Neuroscience·I A FergusonR A Rush
Dec 15, 2006·Nature

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Citations

Jul 9, 2013·Neurological Sciences : Official Journal of the Italian Neurological Society and of the Italian Society of Clinical Neurophysiology·Weiwei LinXiaodong Wang
May 24, 2011·Neurochemical Research·Celine Ullrich, Christian Humpel
Jan 31, 2016·Experimental Neurology·Hassan Al-AliVance P Lemmon
Aug 28, 2015·Molecular Neurobiology·Sareh PandamoozLeila Dargahi

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