Coculturing Bacteria Leads to Reduced Phenotypic Heterogeneities

Applied and Environmental Microbiology
Jasmine HeyseNico Boon

Abstract

Isogenic bacterial populations are known to exhibit phenotypic heterogeneity at the single-cell level. Because of difficulties in assessing the phenotypic heterogeneity of a single taxon in a mixed community, the importance of this deeper level of organization remains relatively unknown for natural communities. In this study, we have used membrane-based microcosms that allow the probing of the phenotypic heterogeneity of a single taxon while interacting with a synthetic or natural community. Individual taxa were studied under axenic conditions, as members of a coculture with physical separation, and as a mixed culture. Phenotypic heterogeneity was assessed through both flow cytometry and Raman spectroscopy. Using this setup, we investigated the effect of microbial interactions on the individual phenotypic heterogeneities of two interacting drinking water isolates. Through flow cytometry we have demonstrated that interactions between these bacteria lead to a reduction of their individual phenotypic diversities and that this adjustment is conditional on the bacterial taxon. Single-cell Raman spectroscopy confirmed a taxon-dependent phenotypic shift due to the interaction. In conclusion, our data suggest that bacterial interaction...Continue Reading

References

Aug 5, 1976·Nature·J L Spudich, D E Koshland
Sep 23, 2000·Journal of Microbiological Methods·G Nebe-von-CaronR A Badley
Apr 23, 2002·Nature Genetics·Ertugrul M OzbudakAlexander van Oudenaarden
Aug 17, 2002·Science·Michael B ElowitzPeter S Swain
Aug 17, 2004·Science·Nathalie Q BalabanStanislas Leibler
Sep 9, 2004·Microbiology and Molecular Biology Reviews : MMBR·Byron F Brehm-Stecher, Eric A Johnson
Mar 29, 2005·Antimicrobial Agents and Chemotherapy·C WiuffB R Levin
Sep 24, 2005·Science·Jonathan M Raser, Erin K O'Shea
May 17, 2006·Trends in Ecology & Evolution·Christine M JessupBrendan J M Bohannan
Jul 18, 2006·Nature Reviews. Microbiology·Simon V Avery
Mar 26, 2008·Nature Genetics·Murat AcarAlexander van Oudenaarden
Apr 17, 2008·Molecular Systems Biology·Jan-Willem VeeningOscar P Kuipers
Aug 23, 2008·Nature·Martin AckermannMichael Doebeli
Feb 18, 2009·Molecular Microbiology·Dawn Fraser, Mads Kaern
Apr 11, 2009·BMC Bioinformatics·Florian HahneRobert Gentleman
Jun 27, 2009·Molecular Microbiology·Claudia AnetzbergerKirsten Jung
Mar 27, 2010·FEMS Microbiology Reviews·Susann Müller, Gerhard Nebe-von-Caron
Aug 21, 2010·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Paul B Rainey, Benjamin Kerr
May 5, 2011·Biotechnology Advances·R Lencastre FernandesK V Gernaey
Jul 17, 2012·Bioinformatics·Sebastian Gibb, Korbinian Strimmer
Nov 28, 2012·The ISME Journal·M Ackermann
Nov 28, 2013·Environmental Microbiology·Karen De RoyNico Boon
May 16, 2014·Journal of the Royal Society, Interface·Lisa GoersKaren M Polizzi
Jan 1, 2015·Proceedings of the National Academy of Sciences of the United States of America·David BerryMichael Wagner
May 15, 2015·Environmental Microbiology·Martin Ackermann, Frank Schreiber
Jul 7, 2015·Nature Reviews. Microbiology·Martin Ackermann
Oct 4, 2015·Applied and Environmental Microbiology·Pedro González-TorresJosefa Antón
Feb 20, 2016·PLoS Computational Biology·Jordi van Gestel, Martin A Nowak
Apr 12, 2016·Frontiers in Microbiology·Sylvie SchulzeJörg Linde
May 6, 2016·Current Opinion in Biotechnology·Yun WangMichael Wagner
Jun 9, 2016·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Kimberly M Davis, Ralph R Isberg
Aug 31, 2016·Nature Microbiology·Frank SchreiberMartin Ackermann

❮ Previous
Next ❯

Citations

Jan 11, 2020·Frontiers in Microbiology·Federica CalabreseHryhoriy Stryhanyuk
Aug 18, 2020·Applied and Environmental Microbiology·Zhong YuFangang Meng
Jan 23, 2021·Applied Microbiology and Biotechnology·Shuaishuai YanQing Liu
Feb 12, 2021·The Science of the Total Environment·Rawil FakhrullinGölnur Fakhrullina
Jan 27, 2021·The Journal of Microbiology·Jin-Kyung HongTae Kwon Lee
Apr 10, 2021·Applied Microbiology and Biotechnology·C G ConacherF F Bauer

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.