PMID: 8955577Nov 1, 1996Paper

Codfish allergy in adults. Specific tests for IgE and histamine release vs double-blind, placebo-controlled challenges

Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology
T K HansenL K Poulsen

Abstract

At present, several in vitro tests for immunoglobulin E (IgE)-mediated food allergy are available. An estimation of the diagnostic accuracy of the various tests used in predicting clinical sensitivity to codfish in a well-characterized allergic material is necessary. To compare the diagnostic value of four specific IgE tests, and histamine release from basophils (HR) in identifying clinical type I allergy to codfish. As a true diagnosis, double-blind, placebo-controlled food challenges (DBPCFC) were employed. Eight clinically codfish-allergic adult patients were investigated together with 30 codfish-tolerant control subjects for evidence of codfish-specific reactivity by Phadebas RAST (PHA), Pharmacia CAP System RAST (CAP), Magic Lite (ML) and HR. To characterize the diagnostic properties of a freshly prepared raw codfish extract, experiments were conducted employing an in-house radioallergosorbent test (RAST), the Maxisorp RAST (MAXI) and HR. Finally, protein profile and IgE-reacting allergens were detected by means of sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and immunoblotting. The sensitivities of HR with commercial extract and the three commercially available specific IgE analyses were 0.83 and 1...Continue Reading

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Citations

Jun 18, 2002·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·B BaderschneiderI J Sanders
Nov 28, 2002·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·C Bindslev-JensenL K Poulsen
Jun 23, 2001·Journal of Chromatography. B, Biomedical Sciences and Applications·L K Poulsen
Feb 1, 1997·Annals of Allergy, Asthma & Immunology : Official Publication of the American College of Allergy, Asthma, & Immunology·T K HansenL K Poulsen
Feb 5, 1998·Allergy·C Bindslev-Jensen, L K Poulsen
Sep 17, 2014·Clinical and Translational Allergy·Karlijn Jg SchulkesAndré C Knulst
Nov 24, 1998·Allergy·C Bindslev-Jensen, L K Poulsen
May 27, 2010·Journal of Human Nutrition and Dietetics : the Official Journal of the British Dietetic Association·E VassilopoulouN G Papadopoulos
Oct 27, 2004·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Tine K HansenCarsten Bindslev-Jensen
Nov 15, 2011·European Journal of Internal Medicine·Giorgio Ciprandi, Ignazio Cirillo
Aug 21, 2010·Otolaryngology--head and Neck Surgery : Official Journal of American Academy of Otolaryngology-Head and Neck Surgery·Asli Sahin-YilmazJacquelynne P Corey
Apr 12, 2001·Allergy·L K PoulsenC Bindslev-Jensen
Oct 28, 2004·Molecular Nutrition & Food Research·Mona H PedersenLars K Poulsen

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