Codon bias and heterologous protein expression

Trends in Biotechnology
Claes GustafssonJeremy Minshull

Abstract

The expression of functional proteins in heterologous hosts is a cornerstone of modern biotechnology. Unfortunately, proteins are often difficult to express outside their original context. They might contain codons that are rarely used in the desired host, come from organisms that use non-canonical code or contain expression-limiting regulatory elements within their coding sequence. Improvements in the speed and cost of gene synthesis have facilitated the complete redesign of entire gene sequences to maximize the likelihood of high protein expression. Redesign strategies are discussed here, including modification of translation initiation regions, alteration of mRNA structural elements and use of different codon biases.

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Related Concepts

Metabolic Process, Cellular
Immune Response
Candida albicans
Streptomyces coelicolor
Caenorhabditis elegans
Amino Acids, I.V. solution additive
MT-TA gene
Proteins, Recombinant DNA
Triplet Codon-amino Acid Adaptor Activity
Genes, Viral

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