Codonopis bulleynana Forest ex Diels inhibits autophagy and induces apoptosis of colon cancer cells by activating the NF-κB signaling pathway

International Journal of Molecular Medicine
Yunpeng LuanYong Cao

Abstract

Despite its favorable clinical efficacy, oxaliplatin‑based chemotherapy frequently results in treatment withdrawal and induces liver damage in colon cancer. Therefore, it is important to develop novel drugs, which can safely and effectively complement or replace the therapeutic effects of oxaliplatin. Codonopis bulleynana Forest ex Diels (cbFeD) has wide range of pharmacological effects, including anticancer effects. In the present study, the anticancer activity of cbFeD and its potential molecular mechanisms were investigated. In vitro, cell counting kit‑8 assays and flow cytometry were used to assess the anti‑proliferation and apoptosis‑promoting activities of cbFeD. Transmission electron microscopy was used to monitor the autophagic vesicles. Immunofluorescence staining was performed to observe the nuclear translocation of p65 and the fluorescence of microtubule‑associated protein 1 light chain 3 (LC3) B‑II. The protein expression levels of p65, inhibitor of nuclear factor (NF)‑κB (IκB) a, LC3B‑I, LC3B‑II and Beclin‑1 were detected using western blot analysis. In vivo, the antitumor effect of cbFeD was assessed in colon cancer‑bearing nude mice as a model. H&E staining and immunohistochemistry (IHC) were performed, with oxal...Continue Reading

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Methods Mentioned

BETA
xenograft
flow cytometry
protein extraction
protein assay
transmission electron microscopy
electron microscopy

Software Mentioned

GraphPad
GraphPad Prism

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