Coencapsulation of hepatocytes with bone marrow mesenchymal stem cells improves hepatocyte-specific functions

Transplantation
Xiao-Lei ShiYi-Tao Ding

Abstract

Hepatocyte transplantation is an alternative to liver transplantation, which is hampered by short survival time and immunorejection. In this study, we investigated the specific functions of hepatocytes coencapsulated with bone marrow mesenchymal stem cells (MSCs) in vitro, and we further examined whether transplantation of coencapsulated hepatocytes and MSCs could enhance the ability of hepatocytes to alleviate acute liver failure in vivo. Rat hepatocytes and MSCs were isolated and coencapsulated by alginate-poly-l-lysine-alginate microencapsulation. Cell functions were monitored during the course of cell culturing. Acute liver failure in rats was induced by d-galactosamine administration. These rats were subjected to intraperitoneal transplantation of coencapsulated hepatocytes with MSCs, encapsulated hepatocytes alone, or empty vehicles after 24 hr. The survival rate and liver functions were assessed. Immunofluorescence microscopy was used for the analysis of coencapsulated cells before and 7 days after transplantation. Hepatocyte-specific functions, including albumin secretion and urea synthesis, were all significantly improved in the coencapsulation group compared with the encapsulated hepatocytes group (P<0.05). Similar tr...Continue Reading

References

Jan 1, 1976·Journal of Immunological Methods·A W Wheeler, P M Hatcher
Jan 1, 1976·Methods in Cell Biology·P O Seglen
May 8, 1999·Transplantation·R P LanzaW L Chick
Aug 6, 2000·Artificial Cells, Blood Substitutes, and Immobilization Biotechnology·Z Liu, T M Chang
Feb 24, 2001·Journal of Hepatology·T M Chang
May 25, 2002·Artificial Cells, Blood Substitutes, and Immobilization Biotechnology·Zun Chang Liu, Thomas Ming Swi Chang
Nov 8, 2002·Biochimica Et Biophysica Acta·Eithan Galun, Jonathan H Axelrod
Mar 30, 2004·Artificial Organs·Thomas Ming Swi Chang
Jul 30, 2005·Journal of Biomedical Materials Research. Part a·Yekaterina S Zinchenko, Robin N Coger
Aug 12, 2005·Journal of Artificial Organs : the Official Journal of the Japanese Society for Artificial Organs·Hiroyuki IjimaKoei Kawakami
Oct 20, 2005·Journal of Bioscience and Bioengineering·Katsuhiro IsodaKiyohito Yagi
Feb 24, 2006·Liver Transplantation : Official Publication of the American Association for the Study of Liver Diseases and the International Liver Transplantation Society·Zun Chang Liu, Thomas Ming Swi Chang
Oct 19, 2006·Biotechnology Annual Review·Zun Chang Liu, Thomas Ming Swi Chang
Mar 6, 2007·Current Gastroenterology Reports·Mouen KhashabPaul Y Kwo
Apr 12, 2007·International Review of Cytology·Naoki Tanimizu, Atsushi Miyajima
Apr 15, 2008·Current Opinion in Gastroenterology·Adnan Said, Michael R Lucey
May 22, 2008·Tissue Engineering. Part C, Methods·Hiroshi MizumotoToshihisa Kajiwara
Jun 24, 2008·Biotechnology and Bioengineering·Rebecca N MoorePrabhas V Moghe
Jan 10, 2009·Artificial Cells, Blood Substitutes, and Immobilization Biotechnology·Zun Chang Liu, Thomas M S Chang
Jan 13, 2009·Transplantation·Bing HanBo Qu

❮ Previous
Next ❯

Citations

May 10, 2011·Tissue Engineering. Part a·Kartik SubramanianWei-Shou Hu
Jun 22, 2012·BioDrugs : Clinical Immunotherapeutics, Biopharmaceuticals and Gene Therapy·Argia AcarreguiRosa M Hernández
Oct 20, 2010·Expert Opinion on Biological Therapy·Satya PrakashArghya Paul
Dec 20, 2011·Journal of Healthcare Engineering·Wujie Zhang, Xiaoming He
Aug 16, 2012·Hepatobiliary & Pancreatic Diseases International : HBPD INT·Xu-Bo Wu, Ran Tao
Dec 6, 2011·Liver Transplantation : Official Publication of the American Association for the Study of Liver Diseases and the International Liver Transplantation Society·Yue YuScott L Nyberg
Oct 15, 2013·Liver International : Official Journal of the International Association for the Study of the Liver·Mónica L LópezUrsula Matte
Nov 7, 2012·Cell Transplantation·Yuan-Sheng LiTzyy-Wen Chiou
Mar 27, 2018·Journal of Immunology Research·Elisa MontanariLeo H Buhler
May 9, 2012·The International Journal of Artificial Organs·Liyuan QiuChangyong Wang
Jan 6, 2016·Biomedicines·Clara NicolasScott L Nyberg
Oct 5, 2019·Current Medicinal Chemistry·Anjum MahmoodAnand S Srivastava
Sep 12, 2017·International Journal of Pharmaceutics·Tania Belen Lopez-MendezGorka Orive

❮ Previous
Next ❯

Related Concepts

Related Feeds