Coenzyme Q6 and iron reduction are responsible for the extracellular ascorbate stabilization at the plasma membrane of Saccharomyces cerevisiae.

The Journal of Biological Chemistry
C Santos-OcañaP Navas

Abstract

Yeast plasma membrane contains an electron transport system that maintains ascorbate in its reduced form in the apoplast. Reduction of ascorbate free radical by this system is comprised of two activities, one of them dependent on coenzyme Q6 (CoQ6). Strains with defects in CoQ6 synthesis exhibit decreased capacity for ascorbate stabilization compared with wild type or with atp2 or cor1 respiratory-deficient mutant strains. Both CoQ6 content in plasma membranes and ascorbate stabilization were increased during log phase growth. The addition of exogenous CoQ6 to whole cells resulted in its incorporation in the plasma membrane, produced levels of CoQ6 in the coq3 mutant strain that were 2-fold higher than in the wild type, and increased ascorbate stabilization activity in both strains, although it was higher in the coq3 mutant than in wild type. Other antioxidants, such as benzoquinone or alpha-tocopherol, did not change ascorbate stabilization. The CoQ6-independent reduction of ascorbate free radical was not due to copper uptake, pH changes or to the presence of CoQ6 biosynthetic intermediates, but decreased to undetectable levels when coq3 mutant strains were cultured in media supplemented with ferric iron. Plasma membrane CoQ6 ...Continue Reading

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Citations

Jul 23, 1999·BioFactors·J R Schultz, C F Clarke
Oct 24, 2002·Biochimica Et Biophysica Acta·Ruth A Hagerman, Richard A Willis
Mar 7, 2001·Antioxidants & Redox Signaling·J M Villalba, P Navas
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Feb 26, 2009·Aging Cell·María Jiménez-HidalgoPlácido Navas
Jan 29, 2014·IUBMB Life·Isabel González-MariscalCarlos Santos-Ocaña
Mar 13, 2003·Redox Report : Communications in Free Radical Research·Jennifer D Ly, Alfons Lawen
Jun 25, 2015·Biogerontology·Guillermo López-LluchPlácido Navas
Dec 29, 2004·Biochimica Et Biophysica Acta·Daniel J M Fernández-AyalaPlácido Navas
Jan 1, 2004·The Journal of Biological Chemistry·Suzie W BabaCatherine F Clarke
Jan 15, 2002·The Journal of Biological Chemistry·Carlos Santos-OcañaCatherine F Clarke
Mar 29, 2005·The Journal of Biological Chemistry·Beth MarboisCatherine F Clarke

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