Abstract
Biofilm formation and efflux pumps (EPs) correlation play a critical role in the pathogenicity and antibiotic resistance of Pseudomonas aeruginosa. In this study, biofilm formation and EP's collaborative role in clinical isolates of P. aeruginosa infection were investigated. Eighty-six (86) P. aeruginosa isolates were collected from different clinical specimens and were confirmed using different biochemical tests. The formation of biofilm was investigated by using a crystal violet assay. Also, EP genes were identified by the PCR method. Based on the results, gentamicin-resistant (n = 50, 66.29%) and ciprofloxacin-resistant (n = 61, 69.66%) strains were the most frequent and colistin (n = 1, 1.12%) and ceftazidime (n = 12, 7.86%) resistant strains were the least prevalent. Furthermore, 22 isolates (31.42%) were MDR, and 11 isolates (12.35%) were XDR strains. Also, 19 isolates (22.47%) were classified as strong biofilm, 29 isolates (21.34%) as moderate biofilm, and 3 (11.23%) isolates as weak biofilm producers. The distribution of the EP genes was as follows: mexA (n = 44, 34.83%), mexB (n = 33, 32.58%), oprM (n = 59, 29.21%), oprD (n = 61, 30.33%), tetA (n = 22, 25.58%), tetR (n = 19, 22.09%), and emrE (n = 21, 24.41%). However,...Continue Reading
References
Aug 15, 2009·Drugs·Xian-Zhi Li, Hiroshi Nikaido
May 12, 2012·BMC Microbiology·Shu MinagawaNaomasa Gotoh
Feb 6, 2013·Virulence·Sara M Soto
Aug 14, 2013·Antimicrobial Agents and Chemotherapy·C RumboUNKNOWN Spanish Network for Research in Infectious Diseases(REIPI)
Dec 4, 2013·Antimicrobial Agents and Chemotherapy·B Eleazar Cohen
Apr 9, 2015·The Journal of Antibiotics·Katsumi ShigemuraMasato Fujisawa
Mar 13, 2016·BMC Microbiology·Thea S B MøllerJohn E Olsen
Jul 28, 2016·Drug Resistance Updates : Reviews and Commentaries in Antimicrobial and Anticancer Chemotherapy·Bryan D Schindler, Glenn W Kaatz
Nov 22, 2016·Critical Reviews in Microbiology·Joana AzeredoClaus Sternberg
Jan 8, 2017·Applied and Environmental Microbiology·Mia Mae KiamcoHaluk Beyenal
Sep 14, 2017·Scientific Reports·Giordano RampioniLivia Leoni
Feb 8, 2018·The Kaohsiung Journal of Medical Sciences·Nandagopal MuruganHajib NarahariRao Madhavan
Mar 6, 2018·The Journal of Antimicrobial Chemotherapy·Ilyas AlavKhondaker Miraz Rahman
Jun 7, 2018·Infection and Drug Resistance·Joseph L KutiDavid P Nicolau
Jun 16, 2018·BMC Complementary and Alternative Medicine·Maryam Meskini, Davoud Esmaeili
Aug 25, 2018·Microbial Drug Resistance : MDR : Mechanisms, Epidemiology, and Disease·Virginio CepasSara M Soto
Jan 25, 2019·Scientific Reports·Anuja Malgaonkar, Mrinalini Nair
Feb 13, 2019·The Journal of Antimicrobial Chemotherapy·Astrid PérezGermán Bou
Apr 11, 2019·Revista Do Instituto De Medicina Tropical De São Paulo·Hectorina RodulfoMarcos De Donato
May 3, 2019·Scientific Reports·Ross PallettLindsay J Marshall
May 17, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Cheng-Hong YangLi-Yeh Chuang
Jul 28, 2019·Scientific Reports·Gertrudis HornaJoaquim Ruiz
Jul 8, 2020·Membranes·Saulo R TintinoValdir Q Balbino
Sep 7, 2020·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Hamed TahmasebiMohammad Reza Arabestani
Sep 15, 2020·Infection and Drug Resistance·Hamed TahmasebiMohammad Reza Arabestani