Coexpression of a constitutively active plasma membrane calcium pump with GFP identifies roles for intracellular calcium in controlling cell sorting during morphogenesis in Dictyostelium

Developmental Biology
A B CubittR A Firtel

Abstract

To examine the potential role of calcium in regulating Dictyostelium development, we reduced free cytosolic and total cell Ca2+ in Dictyostelium cells by expressing a constitutively active form of a human erythrocyte plasma membrane calcium pump. The pump-expressing cells lacked a thapsigargin-mediated increase in cytoplasmic calcium, consistent with a reduced level of total cellular Ca2+. During aggregation, the cells initially formed a large number of aggregation centers, many of which coalesced to form mounds that were smaller than those of wild-type cells, and the cells did not exhibit the normal formation of elongated aggregation streams. The majority of the mounds either arrested at this stage with the formation of small protrusions or formed very aberrant finger-like structures, indicating an essential role for cellular calcium in morphogenesis. We used pump and wild-type cells differentially labeled by expressing different wavelength (green and blue) forms of green fluorescent protein and three-dimensional (3-D) reconstruction of serial fluorescent imaging to visualize the movement of pump and wild-type cells within the aggregate. The results showed that the pump cells exhibited very aberrant cell movement and sorting w...Continue Reading

References

Aug 1, 1992·The Biochemical Journal·H P AdamoJ T Penniston
Sep 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·D TraynorJ G Williams
Feb 11, 1992·Journal of the Optical Society of America. A, Optics and Image Science·C PrezaJ G McNally
Jan 1, 1990·Genes & Development·R A Firtel, A L Chapman
Mar 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·S K Mann, R A Firtel
Jul 13, 1989·Nature·K A JermynJ G Williams
Feb 1, 1985·Developmental Biology·K M Devine, W F Loomis
Jun 1, 1982·Developmental Biology·J A Schmidt, W F Loomis
Sep 1, 1982·Developmental Biology·J Sternfeld, C N David
Aug 1, 1995·Current Biology : CB·F Siegert, C J Weijer
Dec 20, 1994·Proceedings of the National Academy of Sciences of the United States of America·R HeimR Y Tsien
Jan 1, 1994·Progress in Nucleic Acid Research and Molecular Biology·J Williams, A Morrison
Aug 1, 1994·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Y Kubohara, K Okamoto
Feb 11, 1994·Science·M ChalfieD C Prasher
Dec 18, 1995·Experientia·P C NewellJ D Gross
Nov 1, 1995·Trends in Biochemical Sciences·A B CubittR Y Tsien
Feb 1, 1996·Trends in Genetics : TIG·M Y ChenP N Devreotes
Oct 1, 1996·The Biochemical Journal·D MalchowC Schlatterer
Feb 1, 1997·Experimental Cell Research·M AzharV Nanjundiah

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Citations

Mar 3, 2012·Differentiation; Research in Biological Diversity·Yekaterina Poloz, Danton H O'Day
May 19, 2012·Differentiation; Research in Biological Diversity·Yekaterina Poloz, Danton H O'Day

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