Coexpression of adrenomedullin and its receptors in the reproductive system of the rat: effects on steroid secretion in rat ovary

Biology of Reproduction
Yuk-Yin LiWai Sum O

Abstract

The present study demonstrates the expression of adrenomedullin (ADM) in the reproductive system of the female rat and its effect on the secretion of estradiol and progesterone. Ovarian ADM and Adm mRNA levels were decreased at estrus, whereas oviductal Adm mRNA levels were low at proestrus. Both tissues were shown to coexpress mRNAs encoding the calcitonin receptor-like receptor and receptor activity-modifying protein 1 (Ramp1), Ramp2, and Ramp3. Gel filtration chromatography of ovarian extracts showed two peaks, with the predominant one eluting at the position of authentic rat ADM (1-50) at estrus and at the position of ADM precursor at diestrus. Positive ADM immunostaining was localized in the granulosa and theca cells of the follicle and corpora lutea of the ovary. Adrenomedullin inhibited FSH-induced estradiol secretion in 2-day-old follicles and also suppressed eCG-stimulated progesterone release in corpora lutea. The inhibitory effect of ADM on the follicles and the corpora lutea was abolished by calcitonin gene-related peptide (8-37) and ADM (22-52), respectively. The presence of ADM and the gene expression of ADM and its receptor components in the female reproductive system suggest a paracrine effect of ADM on ovarian ...Continue Reading

References

Nov 1, 1989·Quarterly Journal of Experimental Physiology : an International Journal of the Physiological Society·A J NimmoJ F Morrison
Jan 1, 1984·Endocrine Reviews·R P Jansen
Oct 27, 1995·The Journal of Biological Chemistry·S KapasA J Clark
Jul 30, 1993·Biochemical and Biophysical Research Communications·K KitamuraT Eto
Sep 15, 1993·Biochemical and Biophysical Research Communications·J SakataT Eto
Apr 30, 1993·Biochemical and Biophysical Research Communications·K KitamuraT Eto
May 16, 1998·Endocrinology·V A Cameron, A M Fleming
Aug 17, 1999·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·N JiménezL M Montuenga
Mar 10, 2000·Regulatory Peptides·E MarinoniE V Cosmi
Jul 19, 2000·Fertility and Sterility·K AbeK Kangawa
Jan 27, 2004·Journal of Molecular Neuroscience : MN·Debbie L HayDavid R Poyner
Jul 2, 2004·Biology of Reproduction·Josthna PenchalaneniChandrasekhar Yallampalli
Dec 21, 2004·Angiogenesis·Caroline WilsonMargaret C P Rees
May 24, 2005·European Journal of Obstetrics, Gynecology, and Reproductive Biology·Emanuela MarinoniErmelando V Cosmi
Sep 19, 2006·The Journal of Clinical Investigation·Manyu LiKathleen M Caron

❮ Previous
Next ❯

Citations

Jun 25, 2013·Archives of Gynecology and Obstetrics·Poonam RanaFiroz Anwar
Jul 16, 2013·Reproductive Biology and Endocrinology : RB&E·Ken-Go HayashiToru Takahashi
Aug 10, 2011·Reproductive Biology and Endocrinology : RB&E·Lei LiFai Tang
Dec 22, 2010·Reproduction : the Official Journal of the Society for the Study of Fertility·S B LiaoW S O
Nov 15, 2011·Biochemical and Biophysical Research Communications·Yuuki HiradateEimei Sato
Jun 20, 2012·Systems Biology in Reproductive Medicine·Su Bin LiaoWai Sum O
Oct 7, 2016·PloS One·Liza Margareth Medeiros de Carvalho SousaPaula de Carvalho Papa
Mar 30, 2012·The Journal of Clinical Endocrinology and Metabolism·S B LiaoW S O
Jan 10, 2018·Frontiers in Physiology·Sonia Martínez-HerreroAlfredo Martínez
Dec 13, 2018·PloS One·Yvette Audrey Assouni MindjahEmile Telesphore Mboudou

❮ Previous
Next ❯

Methods Mentioned

BETA
PCR
electrophoresis
protein assay
gel filtration
Gel

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.