Coexpression of molecular chaperone enhances activity and export of organophosphorus hydrolase in Escherichia coli

Biotechnology Progress
Dong Gyun KangHyung Joon Cha

Abstract

Periplasmic secretion has been used in attempts to construct an efficient whole-cell biocatalyst with greatly reduced diffusion limitations. Previously, we developed recombinant Escherichia coli that express organophosphorus hydrolase (OPH) in the periplasmic space using the twin-arginine translocation (Tat) pathway to degrade environmental toxic organophosphate compounds. This system has the advantage of secreting protein into the periplasm after folding in the cytoplasm. However, when OPH was expressed with a Tat signal sequence in E. coli, we found that the predominant OPH was an insoluble premature form in the cytoplasm, and thus, the whole-cell OPH activity was significantly lower than its cell lysate activity. In this work, we, for the first time, used a molecular chaperone coexpression strategy to enhance whole-cell OPH activity by improving the periplasmic translocation of soluble OPH. We found that the effect of GroEL-GroES (GroEL/ES) assistance on the periplasmic localization of OPH was secretory pathway dependent. We observed a significant increase in the amount of soluble mature OPH when cytoplasmic GroEL/ES was expressed; this increase in the amount of mature OPH might be due to enhanced OPH folding in the cytoplas...Continue Reading

References

May 1, 1988·Journal of Bacteriology·C S McDanielJ R Wild
Mar 1, 1982·Analytical Biochemistry·H Felix
Mar 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·K TillyC Georgopoulos
Mar 15, 1996·Science·G Schatz, B Dobberstein
Jan 1, 1996·Applied Biochemistry and Biotechnology·S C Hung, J C Liao
Nov 1, 1996·Molecular Microbiology·B C Berks
Aug 19, 2000·Nature·J C SamuelsonR E Dalbey
Feb 15, 2001·Biotechnology Progress·M ShimazuW Chen
Apr 20, 2001·Molecular Microbiology·I J OresnikR J Turner
Apr 25, 2001·Applied and Environmental Microbiology·M A KaderbhaiD C Lamb
May 25, 2001·Nature Structural Biology·A J DriessenC van der Does
May 2, 2003·Proceedings of the National Academy of Sciences of the United States of America·Matthew P DeLisaGeorge Georgiou
Feb 24, 2004·Frontiers in Bioscience : a Journal and Virtual Library·Alberto J L MacarioEverly Conway de Macario
Aug 4, 2004·Methods in Enzymology·Grazyna E Sroga, Jonathan S Dordick
Oct 29, 2005·Journal of Biotechnology·Si-Yu LiSung-Chyr Lin
Nov 28, 2006·Frontiers in Bioscience : a Journal and Virtual Library·Alberto J L Macario, Everly Conway de Macario
May 7, 2009·FEMS Microbiology Reviews·Peter A Lund
Sep 15, 2010·Biotechnology Advances·Carla C C R de Carvalho

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Citations

Aug 15, 2015·Journal of Environmental Science and Health. Part. B, Pesticides, Food Contaminants, and Agricultural Wastes·Meng LiLixin Ma
Sep 5, 2012·Biosensors & Bioelectronics·Chang Sup KimHyung Joon Cha
Nov 26, 2013·Biochimica Et Biophysica Acta·Marie-Pierre Castanié-CornetPierre Genevaux

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