PMID: 9655598Jul 9, 1998Paper

Coexpression of phospholipase A2 isoforms in rat striatal astrocytes

Neuroscience Letters
P ZanassiS Schinelli

Abstract

The expression and activity of phospholipase A2 (PLA2) isoforms were investigated in primary cultures of striatal astrocytes. The calcium ionophore A23187 together with the protein kinase C activator phorbol ester was the most potent stimulus in eliciting [3H]arachidonic acid release in the extracellular medium. Reverse transcription coupled to polymerase chain reaction (RT-PCR) showed the presence of the 85 kDa cytosolic PLA2 mRNA and the 14 kDa secretory PLA2 mRNA in untreated astrocytes. Immunoblot experiments with isoform-specific antibodies showed the presence of the cytosolic PLA2 in untreated astrocytes, while the secretory PLA2 was detected only in lipopolysaccharide-treated astrocytes. These data suggest that the two PLA2 isoforms expressed in striatal astrocytes might play different roles in cellular processes mediated by astrocytes.

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Citations

Oct 20, 1999·Journal of Molecular Neuroscience : MN·W Y OngA A Farooqui
Nov 19, 2003·Prostaglandins, Leukotrienes, and Essential Fatty Acids·J XuG Y Sun
Jul 16, 2004·Prostaglandins, Leukotrienes, and Essential Fatty Acids·Akhlaq A Farooqui, Lloyd A Horrocks
Sep 10, 2016·Genetic Testing and Molecular Biomarkers·Shicheng LiuHuiyi Jiang
May 30, 2015·Journal of Lipid Research·Sasanka RamanadhamXiaoyong Lei
Dec 6, 2003·Journal of Lipid Research·Grace Y SunAgnes Simonyi
Jul 7, 2015·PloS One·Mandy L Roberts-Crowley, Ann R Rittenhouse
Jun 24, 2004·Brain Research. Brain Research Reviews·Akhlaq A FarooquiLouis Freysz

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