Coexpression of TIGIT and FCRL3 identifies Helios+ human memory regulatory T cells

The Journal of Immunology : Official Journal of the American Association of Immunologists
Khalid Bin DhubanCiriaco A Piccirillo

Abstract

Two distinct subsets of CD4(+)Foxp3(+) regulatory T (Treg) cells have been described based on the differential expression of Helios, a transcription factor of the Ikaros family. Efforts to understand the origin and biological roles of these Treg populations in regulating immune responses have, however, been hindered by the lack of reliable surface markers to distinguish and isolate them for subsequent functional studies. Using a single-cell cloning strategy coupled with microarray analysis of different Treg functional subsets in humans, we identify the mRNA and protein expression of TIGIT and FCRL3 as a novel surface marker combination that distinguishes Helios(+)FOXP3(+) from Helios(-)FOXP3(+) memory cells. Unlike conventional markers that are modulated on conventional T cells upon activation, we show that the TIGIT/FCRL3 combination allows reliable identification of Helios(+) Treg cells even in highly activated conditions in vitro as well as in PBMCs of autoimmune patients. We also demonstrate that the Helios(-)FOXP3(+) Treg subpopulation harbors a larger proportion of nonsuppressive clones compared with the Helios(+)FOXP3(+) cell subset, which is highly enriched for suppressive clones. Moreover, we find that Helios(-) cells ...Continue Reading

References

Aug 9, 2001·Proceedings of the National Academy of Sciences of the United States of America·R S DavisM D Cooper
Mar 26, 2005·Proceedings of the National Academy of Sciences of the United States of America·Estelle BettelliMohamed Oukka
Apr 18, 2006·Proceedings of the National Academy of Sciences of the United States of America·Marc A GavinAlexander Y Rudensky
Jul 5, 2006·The Journal of Experimental Medicine·Weihong LiuJeffrey A Bluestone
Feb 15, 2007·PLoS Biology·Stefan FloessJochen Huehn
Mar 7, 2007·Advances in Experimental Medicine and Biology·Götz R A EhrhardtMax D Cooper
Apr 25, 2008·The Journal of Biological Chemistry·Kenji IchiyamaTakashi Kobayashi
May 22, 2008·European Journal of Immunology·Julia K PolanskyJochen Huehn
Dec 17, 2008·Diabetes·Amy L PutnamJeffrey A Bluestone
Jan 29, 2009·Proceedings of the National Academy of Sciences of the United States of America·Noriko KomatsuShohei Hori
May 15, 2009·Proceedings of the National Academy of Sciences of the United States of America·Maha AyyoubDanila Valmori
Jun 6, 2009·The Journal of Immunology : Official Journal of the American Association of Immunologists·Satoshi NagataIra Pastan
Jul 22, 2009·The Journal of Immunology : Official Journal of the American Association of Immunologists·Qi CaiPhilippe Kastner
Oct 3, 2009·Science·Ashutosh ChaudhryAlexander Y Rudensky
Oct 22, 2009·The Journal of Immunology : Official Journal of the American Association of Immunologists·Yuta KochiKazuhiko Yamamoto
Feb 26, 2010·The Journal of Immunology : Official Journal of the American Association of Immunologists·Angela M ThorntonEthan M Shevach
Mar 2, 2010·The Journal of Immunology : Official Journal of the American Association of Immunologists·Louise A SwainsonJoseph M McCune
Mar 17, 2010·Molecular Immunology·Derese GetnetCharles G Drake
Jun 19, 2010·Nature Reviews. Immunology·Shimon SakaguchiDavid A Hafler
Feb 3, 2011·Methods in Molecular Biology·Eva d'Hennezel, Ciriaco A Piccirillo
Mar 4, 2011·The Journal of Immunology : Official Journal of the American Association of Immunologists·Stephanie A McClymontTodd M Brusko
May 18, 2011·The Journal of Immunology : Official Journal of the American Association of Immunologists·Eva d'HennezelCiriaco A Piccirillo
Mar 20, 2012·The Journal of Immunology : Official Journal of the American Association of Immunologists·Ester LozanoDavid A Hafler
May 15, 2012·Journal of Medical Genetics·Eva d'HennezelCiriaco Piccirillo
Jan 30, 2013·The Journal of Immunology : Official Journal of the American Association of Immunologists·Megan E HimmelMegan K Levings
Apr 23, 2013·The Journal of Immunology : Official Journal of the American Association of Immunologists·Maha AyyoubDanila Valmori
Jul 17, 2013·European Journal of Immunology·Fu Jun LiRandall S Davis
Sep 27, 2013·The Journal of Immunology : Official Journal of the American Association of Immunologists·Caroline RaffinMaha Ayyoub

❮ Previous
Next ❯

Citations

Feb 3, 2016·Frontiers in Immunology·Ana Luisa PerdigotoKevan C Herold
Sep 9, 2015·Frontiers in Immunology·Isabelle Le MercierRandolph J Noelle
Nov 8, 2016·Immunological Investigations·Xin-Guang LiuYu Liu
Aug 8, 2017·Science Immunology·Khalid Bin DhubanCiriaco A Piccirillo
Sep 8, 2017·American Journal of Physiology. Renal Physiology·Rahul Sharma, Gilbert R Kinsey
Oct 28, 2017·Journal of Translational Medicine·Jeong-Hee Yang, Seok-Chan Eun
Jan 31, 2018·Frontiers in Immunology·Anke FuchsPiotr Trzonkowski
Aug 5, 2017·Immunotherapy·Xuehui HeIrma Joosten
May 9, 2018·Expert Opinion on Therapeutic Targets·Davood RostamzadehMahdi Shabani
Nov 19, 2015·Molecular Oncology·Anna ŚledzińskaSergio A Quezada
Feb 28, 2018·Clinical & Translational Immunology·Audrey MohrMakoto Miyara
Jan 15, 2019·Leukemia & Lymphoma·Florian AnzengruberEmmanuella Guenova
Mar 14, 2019·Clinical and Experimental Immunology·M AttiasC A Piccirillo
Sep 24, 2019·Antibodies·Steven W de TaeyeGestur Vidarsson
Feb 24, 2016·Scandinavian Journal of Immunology·Patricia Ramos-RamírezApostolos Bossios
May 6, 2020·The Journal of Immunology : Official Journal of the American Association of Immunologists·Rianne OpsteltenDerk Amsen
May 8, 2015·Journal of Neuroimmune Pharmacology : the Official Journal of the Society on NeuroImmune Pharmacology·Laurine Legroux, Nathalie Arbour
Sep 8, 2015·Oncotarget·Eyad ElkordBelal Chaudhary
Apr 15, 2016·The Journal of Immunology : Official Journal of the American Association of Immunologists·Huifang LiMate Tolnay
Nov 3, 2015·World Journal of Gastroenterology : WJG·James D Lord
Jul 22, 2018·Nature Communications·Paloma RiquelmeJames A Hutchinson
Oct 22, 2019·European Journal of Immunology·Andrea CossarizzaArturo Zychlinsky
May 23, 2015·The Journal of Immunology : Official Journal of the American Association of Immunologists·Christopher A FuhrmanTodd M Brusko
Aug 6, 2019·Frontiers in Immunology·Khalid Bin DhubanCiriaco A Piccirillo
Jan 21, 2020·Frontiers in Immunology·Manolo SambucciGiovanna Borsellino
May 1, 2020·Cellular and Molecular Life Sciences : CMLS·Ke Wang, Wenxian Fu
Apr 10, 2020·Nature Reviews. Immunology·Liliana E Lucca, Margarita Dominguez-Villar
Oct 30, 2020·Frontiers in Immunology·Jin-Hwan HanSybil M Genther Williams
Nov 8, 2020·The Journal of Immunology : Official Journal of the American Association of Immunologists·Yini SunMandy L Ford
Jul 8, 2020·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Zhi-Zhang YangStephen M Ansell

❮ Previous
Next ❯

Related Concepts

Related Feeds

Autoimmune Thyroiditis

Autoimmune thyroiditis is an inflammatory disease of thyroid gland due to autoimmune responses leading to lymphocytic infiltration of the gland. It is characterized by the presence of circulating thyroid antigen-specific T-cells and thyroid autoantibodies. Discover the latest research on autoimmune thyroiditis here.

Bioinformatics in Biomedicine

Bioinformatics in biomedicine incorporates computer science, biology, chemistry, medicine, mathematics and statistics. Discover the latest research on bioinformatics in biomedicine here.

Autoimmune Diseases

Autoimmune diseases occur as a result of an attack by the immune system on the body’s own tissues resulting in damage and dysfunction. There are different types of autoimmune diseases, in which there is a complex and unknown interaction between genetics and the environment. Discover the latest research on autoimmune diseases here.

Related Papers

The Journal of Immunology : Official Journal of the American Association of Immunologists
Megan E HimmelMegan K Levings
The Journal of Immunology : Official Journal of the American Association of Immunologists
Eva d'HennezelCiriaco A Piccirillo
© 2021 Meta ULC. All rights reserved