Coexpression of voltage-dependent calcium channels Cav1.2, 2.1a, and 2.1b in vascular myocytes

Hypertension
D AndreasenP B Hansen

Abstract

Voltage-dependent Ca2+ channels Cav1.2 (L type) and Cav2.1 (P/Q type) are expressed in vascular smooth muscle cells (VSMCs) and are important for the contraction of renal resistance vessels. In the present study we examined whether native renal VSMCs coexpress L-, P-, and Q-type Ca2+ currents. The expression of both Cav2.1a (P-type) and Cav2.1b (Q-type) mRNA was demonstrated by RT-PCR in renal preglomerular vessels from rats and mice. Immunolabeling was performed on A7r5 cells, renal cryosections, and freshly isolated renal VSMCs with anti-Cav1.2 and anti-Cav2.1 antibodies. Conventional and confocal microscopy revealed expression of both channels in all of the smooth muscle cells. Whole-cell patch clamp on single preglomerular VSMCs from mice showed L-, P-, and Q-type currents. Blockade of the L-type currents by calciseptine (20 nmol/L) inhibited 35.6+/-3.9% of the voltage-dependent Ca2+ current, and blocking P-type currents (omega-agatoxin IVA 10 nmol/L) led to 20.2+/-3.0% inhibition, whereas 300 nmol/L of omega agatoxin IVA (blocking P/Q-type) inhibited 45.0+/-7.3%. In rat aortic smooth muscle cells (A7r5), blockade of L-type channels resulted in 28.5+/-6.1% inhibition, simultaneous blockade of L-type and P-type channels inhi...Continue Reading

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Citations

Sep 2, 2008·Clinical and Experimental Pharmacology & Physiology·Manuel F Navarro-GonzalezCaryl E Hill
Jul 16, 2010·American Journal of Physiology. Renal Physiology·Zhong ZhangThomas L Pallone
Aug 26, 2010·Physiological Genomics·Steven A Fisher
Jul 16, 2013·The FEBS Journal·Olga KudryavtsevaVladimir V Matchkov
Feb 20, 2007·Circulation Research·Koichi HayashiTakao Saruta
Dec 25, 2010·Stroke; a Journal of Cerebral Circulation·Ellen KnierimMarkus Schuelke
Aug 10, 2017·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·Kayi Y ChanAntoinette MaassenVanDenBrink
Feb 25, 2014·American Journal of Physiology. Heart and Circulatory Physiology·Olga KudryavtsevaChristian Aalkjær
Apr 3, 2015·Physiological Reviews·Mattias CarlströmWilliam J Arendshorst
Dec 7, 2006·American Journal of Physiology. Renal Physiology·T R UhrenholtO Skøtt
Apr 1, 2016·American Journal of Physiology. Renal Physiology·Yiming Zhou, Anna Greka
Sep 19, 2006·American Journal of Physiology. Heart and Circulatory Physiology·Jin ZhangDonald R Matteson

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