Coexpression with Auxiliary β Subunits Modulates the Action of Tefluthrin on Rat Na(v)1.6 and Na(v)1.3 Sodium Channels.

Pesticide Biochemistry and Physiology
Jianguo TanDavid M Soderlund

Abstract

We expressed the rat Na(v)1.3 and Na(v)1.6 sodium channel α subunit isoforms in Xenopus oocytes either alone or with the rat β1 and β2 auxiliary subunits in various combinations and assessed the sensitivity of the expressed channels to resting and use-dependent modification by the pyrethroid insecticide tefluthrin using the two-electrode voltage clamp technique. Coexpression with the β1 and β2 subunits, either individually or in combination, did not affecting the resting sensitivity of Na(v)1.6 channels to tefluthrin. Modification by tefluthrin of Na(v)1.6 channels in the absence of β subunits was not altered by the application of trains of high-frequency depolarizing prepulses. By contrast, coexpression of the Na(v)1.6 channel with the β1 subunit enhanced the extent of channel modification twofold following repeated depolarization. Coexpression of Na(v)1.6 with the β2 subunit also slightly enhanced modification following repeated depolarization, but coexpression of Na(v)1.6 with both β subunits caused enhanced modification following repeated depolarization that was indistinguishable from that found with Na(v)1.6+β1 channels. In contrast to Na(v)1.6, the resting modification by tefluthrin of Na(v)1.3 channels expressed in the a...Continue Reading

References

Nov 1, 1977·The Journal of General Physiology·F Bezanilla, C M Armstrong
May 1, 1996·The Journal of General Physiology·J R BalserG F Tomaselli
Feb 22, 2001·Annual Review of Physiology·A L Goldin
Jan 29, 2002·Toxicology·David M SoderlundMyra L Weiner
Apr 23, 2003·European Journal of Pharmacology·Paul W LenkowskiManoj K Patel
Jul 29, 2003·The Cerebellum·Kristin L Schaller, John H Caldwell
Oct 7, 2004·Biophysical Journal·W Zhou, A L Goldin
Feb 21, 2006·Toxicology and Applied Pharmacology·Kristopher S Silver, David M Soderlund
Jun 10, 2008·Toxicology and Applied Pharmacology·Connie A MeachamTimothy J Shafer
Jul 24, 2010·Pesticide Biochemistry and Physiology·David M Soderlund
Mar 29, 2011·Biochemical and Biophysical Research Communications·Jianguo Tan, David M Soderlund
Oct 4, 2011·Pesticide Biochemistry and Physiology·Jianguo Tan, David M Soderlund

❮ Previous
Next ❯

Citations

Apr 16, 2013·Journal of Bioenergetics and Biomembranes·Debora BaroniOscar Moran

❮ Previous
Next ❯

Related Concepts

Related Feeds

Addiction

This feed focuses mechanisms underlying addiction and addictive behaviour including heroin and opium dependence, alcohol intoxication, gambling, and tobacco addiction.