Cofactor dependence of reduction potentials for [4Fe-4S]2+/1+ in lysine 2,3-aminomutase

Biochemistry
Glen T Hinckley, Perry A Frey

Abstract

Lysine 2,3-aminomutase (LAM) catalyzes the interconversion of l-lysine and l-beta-lysine by a free radical mechanism. The 5'-deoxyadenosyl radical derived from the reductive cleavage of S-adenosyl-l-methionine (SAM) initiates substrate-radical formation. The [4Fe-4S](1+) cluster in LAM is the one-electron source in the reductive cleavage of SAM, which is directly ligated to the unique iron site in the cluster. We here report the midpoint reduction potentials of the [4Fe-4S](2+/1+) couple in the presence of SAM, S-adenosyl-l-homocysteine (SAH), or 5'-{N-[(3S)-3-aminocarboxypropyl]-N-methylamino}-5'-deoxyadenosine (azaSAM) as measured by spectroelectrochemistry. The reduction potentials are -430 +/- 2 mV in the presence of SAM, -460 +/- 3 mV in the presence of SAH, and -497 +/- 10 mV in the presence of azaSAM. In the absence of SAM or an analogue and the presence of dithiothreitol, dihydrolipoate, or cysteine as ligands to the unique iron, the midpoint potentials are -479 +/- 5, -516 +/- 5, and -484 +/- 3 mV, respectively. LAM is a member of the radical SAM superfamily of enzymes, in which the CxxxCxxC motif donates three thiolate ligands to iron in the [4Fe-4S] cluster and SAM donates the alpha-amino and alpha-carboxylate groups...Continue Reading

References

Sep 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·P J StephensB K Burgess
Sep 18, 1973·Biochemical and Biophysical Research Communications·R Cammack
Dec 1, 1972·Proceedings of the National Academy of Sciences of the United States of America·C W CarterL H Jensen
Feb 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·T A KentE Münck
Jul 18, 1997·Biochemical and Biophysical Research Communications·D Guianvarc'hA Marquet
Apr 24, 2001·Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry·J Cheek, J B Broderick
Feb 28, 2002·Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry·Kirsty S HewitsonMarc Fontecave
May 15, 2003·Archives of Biochemistry and Biophysics·Glen T HinckleyPerry A Frey

❮ Previous
Next ❯

Citations

May 1, 2013·Journal of the American Chemical Society·Bradley M HoverKenichi Yokoyama
Feb 4, 2014·Archives of Biochemistry and Biophysics·Krista A Shisler, Joan B Broderick
Jan 19, 2013·Proceedings of the National Academy of Sciences of the United States of America·Mark W RuszczyckyHung-wen Liu
Apr 24, 2013·Biochemistry·Linlin YangLei Li
Nov 12, 2013·Biochemistry·Rebecca C DriesenerPeter L Roach
Jul 23, 2011·Natural Product Reports·Martin R ChallandPeter L Roach
Mar 1, 2008·Critical Reviews in Biochemistry and Molecular Biology·Perry A FreyFrank J Ruzicka
May 15, 2012·Biochimica Et Biophysica Acta·Daniel P DowlingCatherine L Drennan
Jan 25, 2012·Biochimica Et Biophysica Acta·Benjamin R DuffusJoan B Broderick
Dec 17, 2011·Biochimica Et Biophysica Acta·Mark W RuszczyckyHung-Wen Liu
Jan 31, 2014·Chemical Reviews·Joan B BroderickEric M Shepard
Mar 10, 2009·Current Opinion in Chemical Biology·Kaitlin S DuscheneJoan B Broderick
Feb 13, 2007·Trends in Biochemical Sciences·Susan C Wang, Perry A Frey
Mar 2, 2010·Chembiochem : a European Journal of Chemical Biology·E Neil G MarshLei Li
Dec 17, 2010·Current Opinion in Chemical Biology·Peter L Roach
May 28, 2016·Biochemistry·Eric M ShepardJoan B Broderick
Mar 31, 2016·Chembiochem : a European Journal of Chemical Biology·Wei DingQi Zhang
Sep 6, 2019·Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry·Amanda GalambasEric M Shepard
Apr 11, 2008·The Journal of Biological Chemistry·Yvain NicoletJuan C Fontecilla-Camps
Mar 11, 2015·The Journal of Biological Chemistry·Perry Allen Frey
Apr 1, 2015·Israel Journal of Chemistry·Mark W Ruszczycky, Hung-Wen Liu
Nov 16, 2007·Journal of the American Chemical Society·Kenichi YokoyamaTadashi Eguchi
Jul 10, 2019·Journal of the American Chemical Society·Sven A Miller, Vahe Bandarian

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.