Cofactor effects on the protein folding reaction: acceleration of alpha-lactalbumin refolding by metal ions

Protein Science : a Publication of the Protein Society
Natalia A BushmarinaVincent Forge

Abstract

About 30% of proteins require cofactors for their proper folding. The effects of cofactors on the folding reaction have been investigated with alpha-lactalbumin as a model protein and metal ions as cofactors. Metal ions accelerate the refolding of alpha-lactalbumin by lessening the energy barrier between the molten globule state and the transition state, mainly by decreasing the difference of entropy between the two states. These effects are linked to metal ion binding to the protein in the native state. Hence, relationships between the metal affinities for the intermediate states and those for the native state are observed. Some residual specificity for the calcium ion is still observed in the molten globule state, this specificity getting closer in the transition state to that of the native state. The comparison between kinetic and steady-state data in association with the Phi value method indicates the binding of the metal ions on the unfolded state of alpha-lactalbumin. Altogether, these results provide insight into cofactor effects on protein folding. They also suggest new possibilities to investigate the presence of residual native structures in the unfolded state of protein and the effects of such structures on the prote...Continue Reading

Citations

Jun 1, 2013·Analytical and Bioanalytical Chemistry·Gustavo de Souza PessôaMarco Aurélio Zezzi Arruda
Jun 27, 2007·Proceedings of the National Academy of Sciences of the United States of America·Paul SchandaBernhard Brutscher
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Sep 16, 2014·Frontiers in Bioengineering and Biotechnology·M Kalim Akhtar, Patrik R Jones
Jan 19, 2010·Journal of Molecular Biology·Takashi NakamuraKunihiro Kuwajima
Aug 31, 2016·Nature Microbiology·Jozefien De GeyterSpyridoula Karamanou
Oct 20, 2018·Current Medicinal Chemistry·Juan Zeng, Zunnan Huang
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Apr 5, 2020·International Journal of Molecular Sciences·Agnieszka RodzikBogusław Buszewski
Nov 29, 2011·Journal of Molecular Biology·Alexandre ChenalVincent Forge

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