Cofactor engineering to regulate NAD+ /NADH ratio with its application to phytosterols biotransformation

Microbial Cell Factories
Liqiu SuMin Wang

Abstract

Cofactor engineering is involved in the modification of enzymes related to nicotinamide adenine dinucleotides (NADH and NAD+) metabolism, which results in a significantly altered spectrum of metabolic products. Cofactor engineering plays an important role in metabolic engineering but is rarely reported in the sterols biotransformation process owing to its use of multi-catabolic enzymes, which promote multiple consecutive reactions. Androst-4-ene-3, 17-dione (AD) and androst-1, 4-diene-3, 17-dione (ADD) are important steroid medicine intermediates that are obtained via the nucleus oxidation and the side chain degradation of phytosterols by Mycobacterium. Given that the biotransformation from phytosterols to AD (D) is supposed to be a NAD+-dependent process, this work utilized cofactor engineering in Mycobacterium neoaurum and investigated the effect on cofactor and phytosterols metabolism. Through the addition of the coenzyme precursor of nicotinic acid in the phytosterols fermentation system, the intracellular NAD+/NADH ratio and the AD (D) production of M. neoaurum TCCC 11978 (MNR M3) were higher than in the control. Moreover, the NADH: flavin oxidoreductase was identified and was supposed to exert a positive effect on cofacto...Continue Reading

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Citations

Mar 28, 2018·Journal of Industrial Microbiology & Biotechnology·Jiaheng LiuJianjun Qiao
Sep 10, 2020·Plants·Rituraj BatthHenrik Toft Simonsen
Jul 10, 2021·Current Opinion in Biotechnology·Andrew J BorchertGregg T Beckham

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Methods Mentioned

BETA
genetic modification

Software Mentioned

MNR
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