Cofactors influence the biological properties of infectious recombinant prions

Acta Neuropathologica
Natalia Fernández-BorgesJoaquín Castilla

Abstract

Prion diseases are caused by a misfolding of the cellular prion protein (PrP) to a pathogenic isoform named PrPSc. Prions exist as strains, which are characterized by specific pathological and biochemical properties likely encoded in the three-dimensional structure of PrPSc. However, whether cofactors determine these different PrPSc conformations and how this relates to their specific biological properties is largely unknown. To understand how different cofactors modulate prion strain generation and selection, Protein Misfolding Cyclic Amplification was used to create a diversity of infectious recombinant prion strains by propagation in the presence of brain homogenate. Brain homogenate is known to contain these mentioned cofactors, whose identity is only partially known, and which facilitate conversion of PrPC to PrPSc. We thus obtained a mix of distinguishable infectious prion strains. Subsequently, we replaced brain homogenate, by different polyanionic cofactors that were able to drive the evolution of mixed prion populations toward specific strains. Thus, our results show that a variety of infectious recombinant prions can be generated in vitro and that their specific type of conformation, i.e., the strain, is dependent on ...Continue Reading

References

Oct 1, 1993·British Medical Bulletin·M E Bruce
Jan 1, 1997·Neurobiology of Disease·F YehielyS B Prusiner
Jul 8, 1998·Proceedings of the National Academy of Sciences of the United States of America·P ParchiP Gambetti
Oct 15, 1998·Nature Medicine·J SafarS B Prusiner
Oct 29, 1999·Archives of Virology·P K Nandi, E Leclerc
Sep 6, 2003·The Veterinary Record·S L BenestadB Bratberg
Oct 3, 2003·British Medical Bulletin·C Weissmann, E Flechsig
Oct 3, 2003·British Medical Bulletin·Moira E Bruce
Oct 17, 2003·Nature·Nathan R DeleaultSurachai Supattapone
Apr 8, 2004·Molecular Cell·David L VanikWitold K Surewicz
Apr 27, 2005·Cell·Joaquín CastillaClaudio Soto
May 27, 2005·The Journal of Biological Chemistry·Nathan R DeleaultSurachai Supattapone
Sep 20, 2006·The Journal of Biological Chemistry·Paula SaáClaudio Soto
Sep 23, 2006·Science·Melanie Meyer-LuehmannMathias Jucker
Jan 27, 2007·Biochimica Et Biophysica Acta·Rodrigo MoralesClaudio Soto
May 31, 2007·Proceedings of the National Academy of Sciences of the United States of America·Nathan R DeleaultSurachai Supattapone
Nov 10, 2007·Science·John Collinge, Anthony R Clarke
Dec 14, 2007·Proceedings of the National Academy of Sciences of the United States of America·Sukhvir P MahalCharles Weissmann
Apr 9, 2008·Neurobiology of Aging·Valerie L Sim, Byron Caughey
Sep 19, 2008·The EMBO Journal·Joaquín CastillaClaudio Soto
May 14, 2009·PLoS Pathogens·Marcelo A BarriaClaudio Soto
Dec 4, 2009·PLoS Pathogens·Sina GhaemmaghamiStanley B Prusiner
Dec 17, 2009·Prion·Natalia Fernández-BorgesJoaquín Castilla
Jan 2, 2010·Science·Jiali LiCharles Weissmann
Jan 7, 2010·Acta Neuropathologica·Natallia MakaravaIlia V Baskakov
Mar 23, 2010·The Journal of Biological Chemistry·Jae-Il KimWitold K Surewicz
May 29, 2010·Science·John Collinge
Aug 10, 2010·Annals of Neurology·Wen-Quan ZouPierluigi Gambetti
Oct 15, 2011·EMBO Reports·Charles WeissmannShawn Browning
Feb 15, 2012·Proceedings of the National Academy of Sciences of the United States of America·Joel C WattsStanley B Prusiner
May 16, 2012·Proceedings of the National Academy of Sciences of the United States of America·Nathan R DeleaultSurachai Supattapone
Jun 20, 2012·Proceedings of the National Academy of Sciences of the United States of America·Nathan R DeleaultSurachai Supattapone
Jun 5, 2013·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Nuria Gonzalez-MontalbanIlia V Baskakov
Sep 4, 2013·EMBO Reports·Götz HeilbronnerMathias Jucker
Apr 5, 2014·PLoS Pathogens·Joel C WattsStanley B Prusiner
Jul 2, 2014·Proceedings of the National Academy of Sciences of the United States of America·Jan StöhrStanley B Prusiner

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Citations

Jun 22, 2018·PLoS Pathogens·Elizaveta KatorchaIlia V Baskakov
Mar 6, 2018·Brain Pathology·Patricia Aguilar-CalvoChristina J Sigurdson
Mar 13, 2019·Viruses·Etienne LevavasseurStéphane Haïk
Mar 26, 2019·PLoS Pathogens·Cassandra M BurkeSurachai Supattapone
Aug 4, 2018·Molecular Neurobiology·Edoardo BistaffaGiuseppe Legname
Sep 14, 2018·Acta Neuropathologica Communications·Natallia MakaravaIlia V Baskakov
Jan 17, 2020·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Enric VidalJoaquín Castilla
Apr 18, 2020·Brain : a Journal of Neurology·Ilaria VanniRomolo Nonno
Mar 14, 2018·Proceedings of the National Academy of Sciences of the United States of America·Jatish KumarLuis M Liz-Marzán
Dec 29, 2018·Molecular Neurobiology·Chafik HarrathiJoaquín Castilla
Aug 29, 2019·Scientific Reports·Petar Stefanov KovachevSuparna Sanyal
Oct 31, 2020·Frontiers in Bioengineering and Biotechnology·Giovanni SpagnolliJesús R Requena
Nov 17, 2020·Frontiers in Bioengineering and Biotechnology·Hasier ErañaJoaquín Castilla
Dec 19, 2020·Frontiers in Bioengineering and Biotechnology·Mohammed MoudjouAngélique Igel-Egalon

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