Cofilin-mediated sorting and export of specific cargo from the Golgi apparatus in yeast.

Molecular Biology of the Cell
Amy J CurwinVivek Malhotra

Abstract

The mechanism of cargo sorting at the trans-Golgi network (TGN) for secretion is poorly understood. We previously reported the involvement of the actin-severing protein cofilin and the Ca(2+) ATPase secretory pathway calcium ATPase 1 (SPCA1) in the sorting of soluble secretory cargo at the TGN in mammalian cells. Now we report that cofilin in yeast is required for export of selective secretory cargo at the late Golgi membranes. In cofilin mutant (cof1-8) cells, the cell wall protein Bgl2 was secreted at a reduced rate and retained in a late Golgi compartment, whereas the plasma membrane H(+) ATPase Pma1, which is transported in the same class of carriers, reached the cell surface. In addition, sorting of carboxypeptidase Y (CPY) to the vacuole was delayed, and CPY was secreted from cof1-8 cells. Loss of the yeast orthologue of SPCA1 (Pmr1) exhibited similar sorting defects and displayed synthetic sickness with cof1-8. In addition, overexpression of PMR1 restored Bgl2 secretion in cof1-8 cells. These findings highlight the conserved role of cofilin and SPCA1/Pmr1 in sorting of the soluble secretory proteins at the TGN/late Golgi membranes in eukaryotes.

References

Jan 1, 1975·Methods in Enzymology·A Goldstein, J O Lampen
Nov 8, 1990·Nature·M J Lewis, H R Pelham
Jan 1, 1989·Methods in Cell Biology·J R PringleE W Jones
Sep 20, 1985·Science·R A SmithD T Moir
Mar 1, 1995·The Journal of Cell Biology·T A Vida, S D Emr
Oct 1, 1995·The Journal of Cell Biology·E Harsay, A Bretscher
Jul 21, 1995·The Journal of Biological Chemistry·B J AgnewJ R Bamburg
Aug 1, 1993·The Journal of Cell Biology·T E MorganJ R Bamburg
Jul 3, 1997·Nature·P Lappalainen, D G Drubin
Aug 1, 1997·Molecular Biology of the Cell·J MulhollandD Botstein
Jan 19, 2000·Molecular Biology of the Cell·M J LewisH R Pelham
May 4, 2000·Molecular Biology of the Cell·T S KarpovaJ A Cooper
Jan 25, 2002·The Journal of Cell Biology·Edina Harsay, Randy Schekman
Feb 16, 2002·The EMBO Journal·Sangiliyandi GurunathanJeffrey E Gerst
Jul 23, 2002·Molecular Biology of the Cell·Cecilia J BonangelinoJuan S Bonifacino
Oct 23, 2003·Annual Review of Cell and Developmental Biology·Asa E Y Engqvist-Goldstein, David G Drubin
Jan 28, 2004·Cell·Juan S Bonifacino, Benjamin S Glick
Jun 24, 2004·The Journal of Cell Biology·Sebastien CarrenoDavid G Drubin
Oct 12, 2004·Annual Review of Cell and Developmental Biology·Marcus C S LeeRandy Schekman
Dec 7, 2005·Proceedings of the National Academy of Sciences of the United States of America·Tomasz J ProszynskiChristiane Walch-Solimena
Feb 3, 2006·Molecular Biology of the Cell·Micah RobinsonJeffrey E Gerst

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Citations

Jun 29, 2013·Histochemistry and Cell Biology·Gustavo EgeaEnric Gutiérrez-Martínez
Sep 3, 2014·The Journal of Cell Biology·Christine KienzleJulia von Blume
May 21, 2014·Trends in Cell Biology·Christine Kienzle, Julia von Blume
Aug 26, 2014·Annual Review of Cell and Developmental Biology·Yusong GuoRandy Schekman
Aug 27, 2015·Biomolecules·Kristian Prydz
Dec 26, 2012·The Journal of Cell Biology·Julia von BlumeVivek Malhotra
May 21, 2014·Current Opinion in Cell Biology·Effrosyni Papanikou, Benjamin S Glick
Mar 25, 2015·Current Biology : CB·Gonzalo QuassolloAlfredo Cáceres
May 4, 2016·The Journal of Cell Biology·Alvaro H CrevennaJulia von Blume
Oct 16, 2016·Molecular Microbiology·Z SchultzhausB D Shaw
Apr 20, 2017·International Journal of Molecular Sciences·Dorota Konopka-Postupolska, Greg Clark
Feb 1, 2018·Molecular Biology of the Cell·Mehrshad Pakdel, Julia von Blume
Nov 11, 2017·World Journal of Microbiology & Biotechnology·Lulu SongDongguang Xiao
Feb 5, 2019·Journal of Industrial Microbiology & Biotechnology·Lulu SongDongguang Xiao
Nov 22, 2020·Bioengineering·Svetlana DavydenkoArtyom Morozov

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Methods Mentioned

BETA
GTPase
immunoprecipitation
immunoprecipitations
fluorescence microscopy
glycosylation

Software Mentioned

Odyssey
Prism
GraphPad Prism
EUROSCARF
Leica

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