Coherence-controlled holographic microscopy enabled recognition of necrosis as the mechanism of cancer cells death after exposure to cytopathic turbid emulsion

Journal of Biomedical Optics
Jana CollakovaRadim Chmelík

Abstract

Coherence-controlled holographic microscopy (CCHM) in low-coherence mode possesses a pronounced coherence gate effect. This offers an option to investigate the details of cellular events leading to cell death caused by cytopathic turbid emulsions. CCHM capacity was first assessed in model situations that showed clear images obtained with low coherence of illumination but not with high coherence of illumination. Then, the form of death of human cancer cells induced by treatment with biologically active phospholipids (BAPs) preparation was investigated. The observed overall retraction of cell colony was apparently caused by the release of cell-to-substratum contacts. This was followed by the accumulation of granules decorating the nuclear membrane. Then, the occurrence of nuclear membrane indentations signaled the start of damage to the integrity of the cell nucleus. In the final stage, cells shrunk and disintegrated. This indicated that BAPs cause cell death by necrosis and not apoptosis. An intriguing option of checking the fate of cancer cells caused by the anticipated cooperative effect after adding another tested substance sodium dichloroacetate to turbid emulsion is discussed on grounds of pilot experiments. Such observatio...Continue Reading

Citations

Sep 12, 2015·Journal of Biomedical Optics·Bonghwan ChonJeeseong Hwang
Aug 25, 2017·Journal of Biomedical Optics·Lenka StrbkovaRadim Chmelik
Jan 1, 2020·PloS One·Benedict DiederichRainer Heintzmann
Nov 1, 2015·Journal of Biomedical Optics·Zbynek DostalRadim Chmelik

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