Coherent Feedforward Regulation of Gene Expression by Caulobacter σT and GsrN during Hyperosmotic Stress

Journal of Bacteriology
Matthew TienSean Crosson

Abstract

GsrN is a conserved small RNA that is under transcriptional control of the general stress sigma factor, σT, and that functions as a posttranscriptional regulator of Caulobacter crescentus survival under multiple stress conditions. We have defined features of GsrN structure that determine survival under hyperosmotic stress, and we have applied transcriptomic and proteomic methods to identify regulatory targets of GsrN under hyperosmotic conditions. The 5' end of GsrN, which includes a conserved cytosine-rich stem-loop structure, is necessary for cell survival after osmotic upshock. GsrN both activates and represses gene expression in this stress condition. Expression of an uncharacterized open reading frame predicted to encode a glycine zipper protein, osrP, is strongly activated by GsrN. Our data support a model in which GsrN physically interacts with osrP mRNA through its 5' C-rich stem-loop to enhance OsrP protein expression. We conclude that sigT, gsrN, and osrP form a coherent feedforward loop in which σT activates gsrN and osrP transcription during stress and GsrN activates OsrP protein expression at the posttranscriptional level. This study delineates transcriptional and posttranscriptional layers of Caulobacter gene expr...Continue Reading

References

Jan 1, 1991·Methods in Enzymology·B Ely
Oct 5, 1990·Journal of Molecular Biology·S F AltschulD J Lipman
Feb 13, 2001·FEMS Microbiology Letters·P M SantosS Valla
Mar 17, 2001·Molecular Microbiology·N MajdalaniS Gottesman
Jun 21, 2002·Advances in Microbial Physiology·John D Helmann
Jun 26, 2003·Nucleic Acids Research·Michael Zuker
Sep 1, 1964·Bacteriological Reviews·J S POINDEXTER
Oct 7, 2004·Journal of Bacteriology·Shuo ChenGisela Storz
Mar 11, 2005·Nature Reviews. Microbiology·Timothy L Cover, Steven R Blanke
Sep 13, 2005·Annual Review of Microbiology·Nadim Majdalani, Susan Gottesman
Sep 24, 2005·Proceedings of the National Academy of Sciences of the United States of America·Sanguk KimJames U Bowie
Dec 20, 2005·Molecular Microbiology·Maude Guillier, Susan Gottesman
Dec 1, 2006·Nature·Emanuele G BiondiMichael T Laub
Dec 16, 2006·Nucleic Acids Research·Phuongan DamYing Xu
May 19, 2007·Nature Reviews. Genetics·Uri Alon
Nov 8, 2007·Molecular Microbiology·Cristina E Alvarez-MartinezSuely L Gomes
Apr 23, 2008·Current Protocols in Nucleic Acid Chemistry·David H MathewsMichael Zuker
Nov 8, 2008·Nucleic Acids Research·Fenglou MaoYing Xu
Feb 17, 2009·Proceedings of the National Academy of Sciences of the United States of America·Anne Francez-CharlotJulia A Vorholt
May 18, 2010·Journal of Bacteriology·Melissa E MarksSean Crosson
Apr 16, 2011·PloS One·Leticia BritosLucy Shapiro
Jul 20, 2011·Proceedings of the National Academy of Sciences of the United States of America·Emily B GogolCarol A Gross
Sep 13, 2014·BMC Genomics·María Gómez-LozanoSøren Molin
Jan 15, 2015·Trends in Microbiology·Anne Francez-CharlotJulia A Vorholt
Aug 27, 2015·Proceedings of the National Academy of Sciences of the United States of America·Kai PapenfortJörg Vogel
Oct 8, 2015·Annual Review of Genetics·Aretha FiebigSean Crosson
Jan 29, 2017·Applied and Environmental Microbiology·Klara BojanovičKatherine S Long
Apr 30, 2017·Methods in Molecular Biology·Henrik Nielsen
May 5, 2017·Nucleic Acids Research·Martin MannRolf Backofen
May 18, 2018·Nature·Morgan N PriceAdam M Deutschbauer

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Feb 3, 2021·Current Opinion in Microbiology·Kathrin S Fröhlich, Manuel Velasco Gomariz

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