Cohesin occupancy and composition at enhancers and promoters are linked to DNA replication origin proximity in Drosophila

Genome Research
Michelle PhersonDale Dorsett

Abstract

Cohesin consists of the SMC1-SMC3-Rad21 tripartite ring and the SA protein that interacts with Rad21. The Nipped-B protein loads cohesin topologically around chromosomes to mediate sister chromatid cohesion and facilitate long-range control of gene transcription. It is largely unknown how Nipped-B and cohesin associate specifically with gene promoters and transcriptional enhancers, or how sister chromatid cohesion is established. Here, we use genome-wide chromatin immunoprecipitation in Drosophila cells to show that SA and the Fs(1)h (BRD4) BET domain protein help recruit Nipped-B and cohesin to enhancers and DNA replication origins, whereas the MED30 subunit of the Mediator complex directs Nipped-B and Vtd in Drosophila (also known as Rad21) to promoters. All enhancers and their neighboring promoters are close to DNA replication origins and bind SA with proportional levels of cohesin subunits. Most promoters are far from origins and lack SA but bind Nipped-B and Rad21 with subproportional amounts of SMC1, indicating that they bind cohesin rings only part of the time. Genetic data show that Nipped-B and Rad21 function together with Fs(1)h to facilitate Drosophila development. These findings show that Nipped-B and cohesin are di...Continue Reading

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Citations

Aug 14, 2020·Cells·Silvia Meyer-NavaViviana Valadez-Graham
Jan 16, 2021·International Journal of Molecular Sciences·Olga Kyrchanova, Pavel Georgiev
Sep 16, 2019·Molecular Cell·Seungsoo Kim, Jay Shendure
Mar 11, 2021·Developmental Cell·Granton A Jindal, Emma K Farley
Mar 27, 2021·Trends in Cell Biology·Carlos Perea-ResaMichael D Blower
May 28, 2019·Trends in Genetics : TIG·Dale Dorsett
Jul 3, 2021·International Journal of Molecular Sciences·Jisha AntonyJulia A Horsfield
Aug 14, 2021·Frontiers in Molecular Biosciences·Pablo García-Gutiérrez, Mario García-Domínguez
Sep 1, 2021·Life Science Alliance·Melanie L BaileyPhilip Hieter

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