Cohesion is established during DNA replication utilising chromosome associated cohesin rings as well as those loaded de novo onto nascent DNAs.

ELife
Madhusudhan SrinivasanKim Nasmyth

Abstract

Sister chromatid cohesion essential for mitotic chromosome segregation is thought to involve the co-entrapment of sister DNAs within cohesin rings. Although cohesin can load onto chromosomes throughout the cell cycle, it only builds cohesion during S phase. A key question is whether cohesion is generated by conversion of cohesin complexes associated with un-replicated DNAs ahead of replication forks into cohesive structures behind them, or from nucleoplasmic cohesin that is loaded de novo onto nascent DNAs associated with forks, a process that would be dependent on cohesin's Scc2 subunit. We show here that in S. cerevisiae, both mechanisms exist and that each requires a different set of replisome-associated proteins. Cohesion produced by cohesin conversion requires Tof1/Csm3, Ctf4 and Chl1 but not Scc2 while that created by Scc2-dependent de novo loading at replication forks requires the Ctf18-RFC complex. The association of specific replisome proteins with different types of cohesion establishment opens the way to a mechanistic understanding of an aspect of DNA replication unique to eukaryotic cells.

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Citations

Dec 30, 2020·Genes·Benilde García-de-TeresaSara Frias
Mar 7, 2021·International Journal of Molecular Sciences·Diana SantosFrancesca M Pisani
Apr 4, 2021·International Journal of Molecular Sciences·Ana BoavidaFrancesca M Pisani
Feb 4, 2022·Critical Reviews in Biochemistry and Molecular Biology·Wenya HouHuiqiang Lou

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Datasets Mentioned

BETA
GSE151551

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BETA
acetylation
FACS
ChIP
electrophoresis
ChIP-seq

Software Mentioned

position
FastQC
Wapl
filter
Galaxy
Bowtie2
Filter FASTQ Galaxy
Filter60
py
chr

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