Coil-1 of rod domain of NF-L is essential for its assemblyin vivo

Science in China. Series C, Life Sciences
X TongJ Chen

Abstract

Neurofilaments take highly ordered structures composed of parallel mays of 10 nm filaments linked to each other with frequent cross-bridge. It is composed of three components named NF-L, NF-M and NF-H. NF-L is able to form filamentous network alone in Sf9 cells, while M could not. To identify which domain is essential for the assembly of NF-L, two chimera proteins named ML and MML were constructed: ML was composed of the head domain of NF-M and other domains of NF-L; MML was composed of the head and Coil-1 domains of NF-M and Coil-2 and tail domains of NF-L. ML was not only able to form filaments in Sf9 cells, but also co-assemble with NF-M into parallel filamentous bundles. MML could not assemble into filaments. Thus the Coil-1 domain of NF-L was essential for its assembly.

References

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Jan 1, 1988·Annual Review of Biochemistry·P M Steinert, D R Roop
May 1, 1981·The Journal of Cell Biology·M Willard, C Simon
Sep 1, 1993·The Journal of Cell Biology·M K LeeD W Cleveland

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