Coiled-Coil Antagonism Regulates Activity of Venus Flytrap-Domain-Containing Sensor Kinases of the BvgS Family

MBio
Elodie LesneFrançoise Jacob-Dubuisson

Abstract

Bordetella pertussis controls the expression of its virulence regulon through the two-component system BvgAS. BvgS is a prototype for a family of multidomain sensor kinases. In BvgS, helical linkers connect periplasmic Venus flytrap (VFT) perception domains to a cytoplasmic Per-Arnt-Sim (PAS) domain and the PAS domain to the dimerization/histidine phosphotransfer (DHp) domain of the kinase. The two linkers can adopt coiled-coil structures but cannot do so simultaneously. The first linker forms a coiled coil in the kinase mode and the second in the phosphatase mode, with the other linker in both cases showing an increase in dynamic behavior. The intervening PAS domain changes its quaternary structure between the two modes. In BvgS homologues without a PAS domain, a helical "X" linker directly connects the VFT and DHp domains. Here, we used BvgS as a platform to characterize regulation in members of the PAS-less subfamily. BvgS chimeras of homologues with natural X linkers displayed various regulation phenotypes. We identified two distinct coiled-coil registers in the N- and C-terminal portions of the X linkers. Stable coil formation in the C-terminal moiety determines the phosphatase mode, similarly to BvgS; in contrast, coil fo...Continue Reading

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Citations

Jul 17, 2018·Nature Reviews. Microbiology·Françoise Jacob-DubuissonRudy Antoine
Feb 28, 2019·Proceedings of the National Academy of Sciences of the United States of America·Igor DikiyKevin H Gardner
Jun 22, 2019·Annual Review of Microbiology·Alejandro Buschiazzo, Felipe Trajtenberg
Feb 24, 2021·Journal of Bacteriology·Elian DupréFrançoise Jacob-Dubuisson
Jun 3, 2021·International Journal of Molecular Sciences·Ivan GushchinValentin Gordeliy

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Datasets Mentioned

BETA
AAN69015

Software Mentioned

DrawCoil
CCBUILDER
Gromos
hmmsearch
Jalview
PCOILS
Gromacs
LinCS

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