Coinfections identified from metagenomic analysis of cervical lymph nodes from tularemia patients

BMC Infectious Diseases
Dawn N BirdsellDavid M Wagner

Abstract

Underlying coinfections may complicate infectious disease states but commonly go unnoticed because an a priori clinical suspicion is usually required so they can be detected via targeted diagnostic tools. Shotgun metagenomics is a broad diagnostic tool that can be useful for identifying multiple microbes simultaneously especially if coupled with lymph node aspirates, a clinical matrix known to house disparate pathogens. The objective of this study was to analyze the utility of this unconventional diagnostic approach (shotgun metagenomics) using clinical samples from human tularemia cases as a test model. Tularemia, caused by the bacterium Francisella tularensis, is an emerging infectious disease in Turkey. This disease commonly manifests as swelling of the lymph nodes nearest to the entry of infection. Because swollen cervical nodes are observed from many different types of human infections we used these clinical sample types to analyze the utility of shotgun metagenomics. We conducted an unbiased molecular survey using shotgun metagenomics sequencing of DNA extracts from fine-needle aspirates of neck lymph nodes from eight tularemia patients who displayed protracted symptoms. The resulting metagenomics data were searched for m...Continue Reading

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Citations

Jan 17, 2020·The Journal of Pathology. Clinical Research·Caitlin A SelwayLaura S Weyrich
May 26, 2021·Journal of Medical Virology·Parisa S AghbashHossein B Baghi

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Methods Mentioned

BETA
PCR
PCRs
electrophoresis

Software Mentioned

SURPI
Prinseq
Tablet
MetaGeniE
BWA
SUPRI

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