Coinjection with TLR2 agonist Pam3CSK4 reduces the pathology of leishmanization in mice

PLoS Neglected Tropical Diseases
Lu HuangSusana Mendez

Abstract

Cutaneous leishmaniasis caused by Leishmania major is an emergent, uncontrolled public health problem and there is no vaccine. A promising prophylactic approach has been immunotherapy with Toll-like receptor (TLR) agonists to enhance parasite-specific immune responses. We have previously reported that vaccination of C57BL/6 mice with live L. major plus the TLR9 agonist CpG DNA prevents lesion development and confers immunity to reinfection. Our current study aims to investigate whether other TLR agonists can be used in leishmanization without induction of lesion formation. We found that live L. major plus the TLR2 agonist Pam3CSK4 reduced the pathology in both genetically resistant (C57BL/6) and susceptible (BALB/c) mouse strains. The addition of Pam3CSK4 activated dermal dendritic cells and macrophages to produce greater amounts of proinflammatory cytokines in both mouse strains. Both Th1 and Th17 responses were enhanced by leishmanization with L. major plus Pam3CSK4 in C57BL/6 mice; however, Th17 cells were unchanged in BALB/c mice. The production of IL-17 from neutrophils was enhanced in both strains infected with L. major plus Pam3CSK4. However, the sustained influx of neutrophils in sites of infection was only observed in ...Continue Reading

References

Apr 1, 1993·The Journal of Clinical Investigation·C PirmezR L Modlin
Jul 6, 2000·The Journal of Immunology : Official Journal of the American Association of Immunologists·Y BelkaidD Sacks
Aug 18, 2000·The Journal of Immunology : Official Journal of the American Association of Immunologists·F Tacchini-CottierJ A Louis
Apr 6, 2001·The Journal of Immunology : Official Journal of the American Association of Immunologists·S MéndezD Sacks
Nov 3, 2001·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·I ArevaloA Llanos-Cuentas
Aug 30, 2003·Molecular and Biochemical Parasitology·Ingeborg BeckerArmando Isibasi
Oct 15, 2003·Clinical Microbiology Reviews·S Janssens, R Beyaert
Jul 1, 2004·Comparative Immunology, Microbiology and Infectious Diseases·P Desjeux
Jul 24, 2004·Infection and Immunity·Helton da Costa SantiagoLeda Quercia Vieira
Apr 12, 2005·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·S AntinoriM Corbellino
Apr 24, 2007·Journal of Leukocyte Biology·Mélanie CharmoyFabienne Tacchini-Cottier
Aug 24, 2007·FEMS Immunology and Medical Microbiology·Parul TripathiSita Naik
Jun 5, 2008·The Journal of Immunology : Official Journal of the American Association of Immunologists·Nazzy PakpourPhillip Scott
Feb 24, 2009·The Journal of Immunology : Official Journal of the American Association of Immunologists·Susanna Lopez KostkaEsther von Stebut
Jul 21, 2009·Trends in Parasitology·Anuratha SakthianandeswarenEmanuela Handman
Jul 22, 2009·The Journal of Clinical Investigation·Maira G R PittaAlain Dessein
Oct 7, 2009·Proceedings of the National Academy of Sciences of the United States of America·Simona GalloriniAndreas Wack
Nov 20, 2009·The Journal of Immunology : Official Journal of the American Association of Immunologists·Fernanda O NovaisCamila I de Oliveira
Apr 8, 2010·Critical Reviews in Immunology·Anna U BielinskaJames R Baker
Nov 18, 2010·PloS One·Erico Vinícius de Souza CarmoClara Lúcia Barbiéri
Nov 26, 2010·Dermatologic Clinics·Ron Birnbaum, Noah Craft

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Citations

Jul 21, 2015·Expert Opinion on Therapeutic Targets·Nassim MatinNima Rezaei
Aug 8, 2015·Cellular Microbiology·Prajwal Gurung, Thirumala-Devi Kanneganti
Mar 11, 2017·Frontiers in Microbiology·Laís A SacramentoVanessa Carregaro
Sep 14, 2016·Infection and Immunity·Wenlong ZhangYongguo Cao
Apr 21, 2016·Parasitology·Shazia HoseinLaia Solano-Gallego
Jul 23, 2019·IUBMB Life·Abdollah JafarzadehBhaskar Saha
Oct 30, 2016·Parasite Immunology·M BagirovaB Ergenoglu
Sep 14, 2017·Parasite Immunology·M V SanchezD E Cargnelutti
Aug 3, 2017·Scientific Reports·Thiago Aparecido da SilvaMaria Cristina Roque-Barreira
Nov 22, 2016·Frontiers in Immunology·Begoña Pérez-CabezasAnabela Cordeiro-da-Silva
Jan 24, 2021·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Asal KatebiSoheila Ajdary

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Methods Mentioned

BETA
PCR
flow cytometry
ELISA

Software Mentioned

GraphPad Prism
CellQuest

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