Abstract
Type I collagen is an integral component of granulation tissue and scar, that is highly dependent on TGFβ1, a member of a pro-fibrotic family of cytokines, for its promotion and deposition. Blocking COL1A1 gene transcription obstructs type I collagen synthesis, hindering the progress of granulation tissue deposition and fibrosis. Local injections of a double stranded oligodeoxynucleotide (dsODN) decoy, containing the TGFβ1 regulatory element that is located in the distal promoter of the COL1A1 gene, were investigated in a rat polyvinyl alcohol (PVA) sponge granulation tissue implant model. The effects on the granulation tissue deposition by dsODN decoy therapy were evaluated by the synthesis of types I and III collagens as well as ED-A (cellular) fibronectin. Fluorescently labeled dsODN was used to identify the distribution of the decoy molecules in the sponge implant relative to the observed histological effects. Morphological alterations in cells and changes in the organization of connective tissue were documented and evaluated. Collagen levels were reduced by half in implants treated with 10 nM dsODN decoy compared to scrambled dsODN-treated implants. Histologically, dsODN decoy treated implants had an increased cellular den...Continue Reading
References
Sep 1, 1978·Archives of Surgery·S AriyanT J Krizek
Dec 13, 1996·Cell·H KoyamaR Ross
Apr 12, 2002·The Journal of Biological Chemistry·Anna Astriab FisherRudolph L Juliano
Nov 2, 2002·The British Journal of Dermatology·Y OishiT Noguchi
Jun 5, 2003·Burns : Journal of the International Society for Burn Injuries·Kristine M BombaroShari Honari
Aug 26, 2003·Journal of Cellular Biochemistry·Kenneth R Cutroneo
Oct 16, 2003·Biochemical and Biophysical Research Communications·Jong Deok AhnYoung-Chae Chang
Apr 20, 2004·Journal of Cellular Biochemistry·Kenneth R Cutroneo, Kenneth M Sterling
Sep 2, 2005·International Journal of Dermatology·Yasemin Saray, A Tülin Güleç
Aug 15, 2006·Burns : Journal of the International Society for Burn Injuries·Donald A Hudson, Anthony Renshaw
Aug 18, 2006·Current Opinion in Pediatrics·Alison E Slemp, Richard E Kirschner
Nov 23, 2006·Archives of Facial Plastic Surgery·Douglas LeventhalDavid Reiter
Oct 1, 1994·Wound Repair and Regeneration : Official Publication of the Wound Healing Society [and] the European Tissue Repair Society·K R CutroneoD F Counts
Dec 16, 2006·The Journal of Dermatology·Sung Won WhangKee Yang Chung
Citations
Apr 24, 2012·Journal of Ethnopharmacology·Moorthy GaneshkumarLonchin Suguna
Apr 6, 2013·PloS One·Xiaodong PanGenshan Ma
Nov 12, 2015·PloS One·Adrian M StankiewiczGrzegorz R Juszczak
Jul 4, 2017·Experimental Gerontology·Giovanni CorsettiFrancesco S Dioguardi
Nov 19, 2013·Pharmaceutical Patent Analyst·Olga KhorkovaClaes Wahlestedt
Aug 21, 2012·Scandinavian Journal of Gastroenterology·Linda GillbergPer M Hellström
Sep 22, 2019·Biological Research·Wei Cao, Youping Feng
Jun 25, 2020·International Journal of Molecular Sciences·Andreas BayerJürgen Harder