Colcemid increases the frequency of chromosome abnormalities in human sperm

Cytogenetics and Cell Genetics
C MárquezC Templado

Abstract

The effect of Colcemid on the frequency and type of chromosomal abnormalities in human sperm was investigated. Using the human sperm and zona-free hamster egg fusion technique, penetrated eggs were cultured in the presence or absence of Colcemid. We used two different times of Colcemid treatment: standard Colcemid treatment (Colcemid-5 h) or long Colcemid treatment (Colcemid-17 h). Each Colcemid series had its own control series without Colcemid, thus ensuring that Colcemid was the only significant variable. A total of 771 sperm karyotypes from one normal donor was analyzed: 286 in the Colcemid-5 h series, 262 in the Colcemid-17 h series, and 223 in the two control series. In both Colcemid series there was a significant increase in the frequencies of hypohaploidy vs. hyperhaploidy (9.4% and 7.3% vs. 2.4% and 1.1%, for the Colcemid-5 h and Colcemid-17 h series, respectively), in contrast to those obtained in the control series, in which the frequencies of hypohaploidy and hyperhaploidy were close to the 1:1 relationship (4.9% vs. 4.0%) expected from nondisjunction. There was a significant increase in the frequency of structural abnormalities in both Colcemid series (16.1% and 14.5% for the Colcemid-5 h and Colcemid-17 h series, ...Continue Reading

Citations

Jun 17, 2006·Cloning and Stem Cells·Martha C GómezBetsy L Dresser
May 31, 2013·Molecular Human Reproduction·C TempladoA Estop
Mar 3, 2012·Human Reproduction·L Uroz, C Templado
Feb 14, 2008·Human Reproduction·Laia UrozCristina Templado
Feb 2, 2011·Cytogenetic and Genome Research·C TempladoA Estop
Sep 30, 2005·Cytogenetic and Genome Research·C TempladoJ Benet
Sep 30, 2005·Cytogenetic and Genome Research·A BuweM Schmid

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