Colchicine alters the quantitative and qualitative display of selectins on endothelial cells and neutrophils

The Journal of Clinical Investigation
B N CronsteinG Weissmann

Abstract

Since colchicine-sensitive microtubules regulate the expression and topography of surface glycoproteins on a variety of cells, we sought evidence that colchicine interferes with neutrophil-endothelial interactions by altering the number and/or distribution of selectins on endothelial cells and neutrophils. Extremely low, prophylactic, concentrations of colchicine (IC50 = 3 nM) eliminated the E-selectin-mediated increment in endothelial adhesiveness for neutrophils in response to IL-1 (P < 0.001) or TNF alpha (P < 0.001) by changing the distribution, but not the number, of E-selectin molecules on the surface of the endothelial cells. Colchicine inhibited stimulated endothelial adhesiveness via its effects on microtubules since vinblastine, an agent which perturbs microtubule function by other mechanisms, diminished adhesiveness whereas the photoinactivated colchicine derivative gamma-lumicolchicine was inactive. Colchicine had no effect on cell viability. At higher, therapeutic, concentrations colchicine (IC50 = 300 nM, P < 0.001) also diminished the expression of L-selectin on the surface of neutrophils (but not lymphocytes) without affecting expression of the beta 2-integrin CD11b/CD18. In confirmation, L-selectin expression w...Continue Reading

Associated Clinical Trials

May 18, 2020·Dr. Ali Mostafaie, PhD, Dr. Ali Mostafaie, PhD

References

Aug 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·S A RudolphS E Malawista
Jun 30, 1975·Annals of the New York Academy of Sciences·I Yahara, G M Edelman
Mar 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·B N CronsteinR I Levin
Mar 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·T K KishimotoE C Butcher
Dec 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·M P BevilacquaM A Gimbrone
Sep 1, 1986·The Journal of Clinical Investigation·B N CronsteinR Hirschhorn
Mar 1, 1973·Proceedings of the National Academy of Sciences of the United States of America·R B ZurierG Weissmann
Oct 1, 1973·Proceedings of the National Academy of Sciences of the United States of America·I GoldsteinG Weissmann
Jan 1, 1973·Annual Review of Biochemistry·J B Olmsted, G G Borisy
Dec 15, 1981·Biochemical and Biophysical Research Communications·J WhittakerK G Alberti
Oct 1, 1993·The Journal of Clinical Investigation·C J RobergeP H Naccache
Feb 1, 1993·Arthritis and Rheumatism·B N Cronstein, G Weissmann

❮ Previous
Next ❯

Citations

Jan 1, 1996·International Journal of Clinical & Laboratory Research·G BertonC A Lowell
Feb 14, 2004·Rheumatology International·Eldad Ben-ChetritMicha Levy
Jan 30, 2013·Rheumatology International·Baris Onder PamukDidem Leyla Kozaci
Jan 9, 2007·Clinical Rheumatology·Aşkin AteşHüseyin Tutkak
Jan 5, 2008·Current Pain and Headache Reports·Aryeh M AbelesBruce N Cronstein
Feb 12, 1998·Biochimica Et Biophysica Acta·J D CroxtallR Flower
May 13, 1998·Immunology Today·F Díaz-González, F Sánchez-Madrid
Jun 6, 2000·Advanced Drug Delivery Reviews·S F Hamm-Alvarez
Apr 18, 2001·BJOG : an International Journal of Obstetrics and Gynaecology·E Ben-Chetrit, A Ben-Chetrit
Jun 4, 2008·Annals of Allergy, Asthma & Immunology : Official Publication of the American College of Allergy, Asthma, & Immunology·Matt Morgan, David A Khan
Nov 26, 2009·Immunology and Cell Biology·Ru Liu-Bryan
Sep 5, 2012·Nature Reviews. Rheumatology·Kenneth L RockJiann-Jyh Lai
Aug 1, 1997·Natural Product Reports·K W Bentley
Jul 25, 2000·The British Journal of Dermatology·M W Greaves
Jun 21, 2012·Obstetrical & Gynecological Survey·Sarah Dotters-KatzThomas Price
Dec 10, 1999·American Journal of Reproductive Immunology : AJRI·R OrvietoY Molad
May 26, 1999·Annals of the Rheumatic Diseases·D M ChangM L Chang
Sep 16, 2003·Annals of the Rheumatic Diseases·E Ben-Chetrit, M Levy
Mar 17, 2005·Blood·Kamal D PuriThomas G Diacovo
Jul 6, 2006·Arthritis Research & Therapy·Bruce N Cronstein, Robert Terkeltaub
Nov 24, 2007·BioDrugs : Clinical Immunotherapeutics, Biopharmaceuticals and Gene Therapy·F Perez-RuizA M Beites
Jun 5, 2012·The Journal of Rheumatology·Daria B CrittendenMichael H Pillinger
Mar 17, 2010·Indian Journal of Dermatology, Venereology and Leprology·Chandana Konda, Angoori Gnaneshwar Rao
May 16, 2002·Current Rheumatology Reports·Yair Molad
Jul 22, 2008·Current Rheumatology Reports·George Nuki
Sep 15, 2015·The Journal of Dermatological Treatment·Şule GüngörDilek Canat
May 14, 2011·Postgraduate Medicine·Paul P Doghramji
Oct 27, 2005·The Journal of Dermatological Treatment·C SunderkötterT Luger
Aug 12, 2003·Hypertension in Pregnancy : Official Journal of the International Society for the Study of Hypertension in Pregnancy·Jacob BarYair Molad
Aug 14, 2012·Autoimmunity Reviews·Leonardo PunziFrancesca Oliviero
Feb 16, 2007·The Journal of Investigative Dermatology·Norikatsu MizumotoAkira Takashima
Nov 1, 2008·Seminars in Arthritis and Rheumatism·Robert A Terkeltaub
Jan 26, 2007·Advances in Dermatology·Manop PithukpakornJorge R Toro

❮ Previous
Next ❯

Related Concepts

Related Feeds

Adhesion Molecules in Health and Disease

Cell adhesion molecules are a subset of cell adhesion proteins located on the cell surface involved in binding with other cells or with the extracellular matrix in the process called cell adhesion. In essence, cell adhesion molecules help cells stick to each other and to their surroundings. Cell adhesion is a crucial component in maintaining tissue structure and function. Discover the latest research on adhesion molecule and their role in health and disease here.