Cold acclimation-induced up-regulation of the ribosomal protein L7 gene in the freeze tolerant wood frog, Rana sylvatica

Gene
Shao-Bo WuKenneth B Storey

Abstract

Natural freezing survival by the wood frog, Rana sylvatica, involves multiple organ-specific changes in gene expression. The present study used differential display PCR to find cold-responsive genes in wood frog skin. A cDNA was retrieved from skin that was in higher amounts in cold- versus warm-acclimated frogs. The cDNA was used to probe a wood frog liver cDNA library and retrieve a long sequence that, after the further application of 5'RACE, was shown to encode the full sequence of the ribosomal large subunit protein 7 (RPL7) (GenBank accession number AF175983). Wood frog RPL7 contained 246 amino acids and shared 90% identity with Xenopus laevis RPL7, 82-83% with chicken and zebrafish homologues, and 79% with mammalian RPL7. Multiple binding domains found in human RPL7 showed differing degrees of conservation in the frog protein. Transcript levels of rpl7 were elevated up to 4-fold in skin of cold-acclimated frogs as compared with warm-acclimated animals. Organ-specific responses by rpl7 transcripts also occurred when frogs were given survivable freezing exposures. Transcripts rose by 1.8-3.3 fold in brain and skeletal muscle during freezing but were unaffected in central organs such as liver and heart. Up-regulation of rpl7...Continue Reading

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Citations

Jan 25, 2012·International Journal of Molecular Sciences·Andor J KissJon P Costanzo
Mar 5, 2015·Fish & Shellfish Immunology·Qing-Hui LiuJie Huang
Sep 12, 2008·Journal of Experimental Zoology. Part A, Ecological Genetics and Physiology·Shaobo WuKenneth B Storey
Jul 25, 2015·Cellular and Molecular Life Sciences : CMLS·Hitomi Takada, Akira Kurisaki
Feb 10, 2017·Physiological Reviews·Kenneth B Storey, Janet M Storey
Apr 24, 2018·Cellular and Molecular Life Sciences : CMLS·Hanane Hadj-Moussa, Kenneth B Storey
Jun 4, 2020·Scientific Reports·Melak WeldenegodguadJuha Kantanen
Jul 3, 2021·International Journal of Molecular Sciences·Federico Martinez-SeidelAlexandre Augusto Pereira Firmino

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