PMID: 19912774Dec 1, 1990Paper

Cold and novel environment stress affects AVP mRNA in the paraventricular nucleus, but not the supraoptic nucleus: An in Situ hybridization study

Molecular and Cellular Neurosciences
P Wu, G V Childs

Abstract

The distribution and area of label for arginine vasopressin (AVP) mRNA or peptides were studied in rats exposed to cold or novel environments. In situ hybridization histochemistry was employed to detect AVP mRNA in hypothalamic frozen sections with a 45-mer photobiotinylated oligonucleotide probe. The storage of the peptide in both the hypothalamus and the pituitary was determined by immunohistochemistry. Label for mRNA or peptide was then quantified by the Cue-3 color image analysis system. Exposure to 4 degrees C for 30 min caused a 3.5-fold increase in the label for AVP mRNA in the paraventricular nucleus (PVN) compared with that of control rats. This was correlated with a 2-fold elevation in serum ACTH. In addition, rats exposed to 30 min of a novel, thermoneutral (24 degrees C) environment showed a 1.2- to approximately -2.3-fold enhancement of the label for AVP mRNA in the PVN. In contrast, no changes were seen in the supraoptic nucleus (SON) following exposure to either cold or novel environments. Furthermore, neither stress caused significant changes in the storage of AVP peptide in the PVN, SON, median eminence, and posterior lobe of pituitary. This in vivo study demonstrates that PVN and SON neurons respond differenti...Continue Reading

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Citations

Mar 9, 1999·Alcoholism, Clinical and Experimental Research·C K KimJ Weinberg
Dec 29, 1995·Annals of the New York Academy of Sciences·M S HarbuzS L Lightman
Mar 1, 1993·Trends in Endocrinology and Metabolism : TEM·M F Dallman
Dec 7, 2002·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·Nancy F Cruz, Gerald A Dienel
Oct 6, 2020·Frontiers in Endocrinology·Gwen V ChildsMelanie C MacNicol

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