Cold-induced cysts of the photosynthetic dinoflagellate Lingulodinium polyedrum have an arrested circadian bioluminescence rhythm and lower levels of protein phosphorylation

Plant Physiology
Sougata RoyDavid Morse

Abstract

Dinoflagellates are microscopic, eukaryotic, and primarily marine plankton. Temporary cyst formation is a well-known physiological response of dinoflagellate cells to environmental stresses. However, the molecular underpinnings of cold-induced cyst physiology have never been described. Cultures of the photosynthetic dinoflagellate Lingulodinium polyedrum readily form temporary cysts when placed at low (8°C±1°C) temperature and excyst to form normal motile cells following a return to normal temperature (18°C±1°C). The normal circadian bioluminescence rhythm and the expected changes in Luciferin Binding Protein abundance were arrested in L. polyedrum cysts. Furthermore, after excystment, the bioluminescence rhythm initiates at a time corresponding to zeitgeber 12, independent of the time when the cells encysted. Phosphoprotein staining after two-dimensional polyacrylamide gel electrophoresis, as well as column-based phosphoprotein enrichment followed by liquid chromatography tandem mass spectrometry, showed cyst proteins are hypophosphorylated when compared with those from motile cells, with the most marked decreases found for predicted Casein Kinase2 target sites. In contrast to the phosphoproteome, the cyst proteome is not mark...Continue Reading

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Citations

May 19, 2017·Proceedings of the National Academy of Sciences of the United States of America·Yoriko MurayamaHiroshi Ito
Jun 16, 2016·MSphere·Mathieu BeaucheminDavid Morse
Aug 17, 2019·Microorganisms·Bahareh ZaheriDavid Morse
Aug 28, 2019·Microorganisms·Yali BiWeiwen Zhang
Nov 21, 2017·Proteomics·Sirius P K TseSamuel C L Lo

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