PMID: 11904229Mar 21, 2002Paper

Cold-induced enzyme inactivation: how does cooling lead to pyridoxal phosphate-aldimine bond cleavage in tryptophanase?

Biochimica Et Biophysica Acta
T ErezAbraham H Parola

Abstract

The phenomenon of cold scission or cold lability, which entails a widespread variety of oligomeric enzymes, is still enigmatic. The effect of cooling on the activity and the quaternary structure of the pyridoxal 5'-phosphate (PLP)-dependent enzyme, tryptophanase (Tnase), was studied utilizing single photon counting time-resolved spectrofluorometry. Upon cooling of holo-wild-type (wt) Tnase and its W330F mutant from 25 degrees C to 2 degrees C, a reduction in PLP fluorescence lifetime and rotational correlation time as well as inactivation and dissociation from tetramers to dimers were observed for both enzymes. Fluorescence anisotropy invariably decreased as a consequence of cooling, whether it was accompanied by a slight decrease in activity without significant dissociation, or by a substantial decrease in activity that was associated with either a partial or major dissociation. These results support the suggested conformational change that precedes the PLP-aldimine bond scission. It is proposed that cold inactivation is initiated by the weakening of hydrophobic interactions, leading to conformational changes which are the driving force for the aldimine bond cleavage.

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Citations

Oct 10, 2009·BMC Structural Biology·Anna KoganOrna Almog
Feb 17, 2005·Journal of Cellular Biochemistry·Helena FidlerováIvan Raska
Feb 6, 2013·Biotechnology and Bioengineering·Justyna KuligDörte Rother
Apr 13, 2019·Organic & Biomolecular Chemistry·Noelia Bernardo-GarcíaFederico Gago
Dec 3, 2015·Acta Crystallographica. Section D, Biological Crystallography·Keren GreenOrna Almog

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