Cold Inducible RNA Binding Protein Is Involved in Chronic Hypoxia Induced Neuron Apoptosis by Down-Regulating HIF-1α Expression and Regulated By microRNA-23a

International Journal of Biological Sciences
Xiaoming ChenJingyuan Chen

Abstract

Background: Neuron apoptosis mediated by hypoxia inducible factor 1α (HIF-1α) in hippocampus is one of the most important factors accounting for the chronic hypobaric hypoxia induced cognitive impairment. As a neuroprotective molecule that is up-regulated in response to various environmental stress, CIRBP was reported to crosstalk with HIF-1α under cellular stress. However, its function under chronic hypobaric hypoxia remains unknown.Objective:In this study, we tried to identify the role of CIRBP in HIF-1α mediated neuron apoptosis under chronic hypobaric hypoxia and find a possible method to maintain its potential neuroprotective in long-term high altitude environmental exposure.Methods:We established a chronic hypobaric hypoxia rat model as well as a tissue culture model where SH-SY5Y cells were exposed to 1% hypoxia. Based on these models, we measured the expressions of HIF-1α and CIRBP under hypoxia exposure and examined the apoptosis of neurons by TUNEL immunofluorescence staining and western blot analysis of apoptosis related proteins. In addition, by establishing HIF-1α shRNA and pEGFP-CIRBP plasmid transfected cells, we confirmed the role of HIF-1α in chronic hypoxia induced neuron apoptosis and identified the influence...Continue Reading

Citations

Jan 16, 2019·Journal of Leukocyte Biology·Monowar AzizPing Wang
Nov 15, 2017·Future Science OA·Peng Zhong, He Huang
Jul 5, 2019·Interactive Cardiovascular and Thoracic Surgery·Yongnan LiBingyang Ji
Mar 8, 2020·Journal of Alzheimer's Disease : JAD·Yaowei HuangYafang Hu
Jun 19, 2019·International Journal of Molecular Sciences·Yung-Che ChenMeng-Chih Lin
Feb 26, 2019·Therapeutic Hypothermia and Temperature Management·Travis C Jackson, Patrick M Kochanek
Aug 17, 2021·Frontiers in Cell and Developmental Biology·Elena V MitroshinaMaria V Vedunova
Oct 8, 2021·Metabolic Brain Disease·Xiaoyin WangXunming Ji

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Methods Mentioned

BETA
environmental stress
protein assay
electrophoresis
flow cytometry
transfection
PCR

Software Mentioned

GraphPad Prism
Image J
GraphPad

Related Concepts

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Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis