Cold preservation of pig liver grafts with warm ischemia and pentoxifylline-UW solution

Archives of Medical Research
De-Ke QingXun-Ru Chen

Abstract

We undertook this study to investigate the safe time limits of cold preservation in UW solution of liver grafts subjected to warm ischemia (WI) for 20 min and the changes of the limits when pentoxifylline is added to UW solution. The safe time limit was studied in a simple porcine orthotopic liver transplantation (LTx) model. In donors, livers were subjected to 20 min of WI and subsequent 12-h (group 1, n = 5), 16-h (group 2, n = 5), and 20-h (group 3, n = 3) cold preservation in UW solution, respectively. After the safe time limits were clear, another group (group 4, n = 5) was built to test whether or not the limits can be changed when pentoxifylline is added to UW solution in an unsafe time limit group. All five animals in group 1 survived up to 7 days of the survey endpoint. In group 2, only one animal survived up to the same survey endpoint and all animals in group 3 died within 12 h. The 1-week survival rate of group 1 was significantly higher than the other two groups. Group 1 had a lower level of alanine aminotransferase (ALT) or aspartase aminotransferase (AST) after LTx, less pathological damage, higher concentration of adenosine triphosphate (ATP) and higher microcirculation blood flux in the grafted liver tissue at ...Continue Reading

References

Aug 1, 1992·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·M GotoT Kamada
Nov 1, 1990·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·J MartínkováP Hůlek
Jun 1, 1989·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·O ChazouillèresR Poupon
Jan 1, 1989·International Review of Cytology·K WakeE Wisse
Feb 24, 2001·Transplantation·F OikeP Gianello

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Citations

Oct 1, 2009·Liver Transplantation : Official Publication of the American Association for the Study of Liver Diseases and the International Liver Transplantation Society·Olivier de RougemontPierre-Alain Clavien
Oct 23, 2013·Journal of Investigative Surgery : the Official Journal of the Academy of Surgical Research·Patricia GenovésJosé Miguel Lloris-Carsi
Feb 4, 2010·Current Opinion in Organ Transplantation·Olivier de RougemontPierre-Alain Clavien
Aug 8, 2008·Current Opinion in Organ Transplantation·James V Guarrera, Niaz A Karim
Jul 11, 2014·Current Opinion in Organ Transplantation·Paolo Muiesan, Simon Fisher

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